- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Effects of Gamijinhae-tang (Jiaweizhenke-tang) on Tracheal Smooth Muscle Contraction and Mucin Secretion from Airway Epithelial Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.3, pp.63-75
&
(2008). Effects of Gamijinhae-tang (Jiaweizhenke-tang) on Tracheal Smooth Muscle Contraction and Mucin Secretion from Airway Epithelial Cells. Journal of Korean Medicine, 29(3), 63-75.
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Abstract
Objectives:In the present study, the author intended to investigate whether Gamijinhae-tang (Jiaweizhenke-tang) (GJHT) significantly affects both contractility of tracheal smooth muscle and mucin secretion from airway epithelial cells. Materials and Methods:Effect of GJHT on contractility of isolated tracheal smooth muscle of rabbit was investigated. Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of GJHT to assess the effect of the agent on 3H-mucin secretion. At the same time, confluent NCI-H292 cells were chased for 30 min in the presence of GJHT to assess the effect of the agent on MUC5AC secretion by ELISA. Total elution profiles of control spent media and treatment sample (radioactive mucin) through Sepharose CL-4B column were analyzed. Also, effect of the agent on MUC5AC gene expression in cultured NCI-H292 cells was investigated. Possible cytotoxicities of the agent were assessed by measuring both lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results: (1) GJHT inhibited Ach-induced contraction of isolated tracheal smooth muscle; (2) GJHT significantly increased mucin secretion from cultured HTSE cells. However, it did not affect MUC5AC secretion from NCI-H292 cells, only chiefly affecting the 'mucin' secretion; (3) GJHT did not significantly affect the expression levels of MUC5AC gene in cultured NCI-H292 cells; (4) GJHT did not significantly inhibit the survival and proliferation of NCI-H292 cells. However, it slightly increased LDH release from HTSE cells. Conclusion:The author suggests that effects of GJHT with their components should be further investigated and it is valuable to find, from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.
- keywords
- Airway, mucus, mucin, Gamijinhae-tang (Jiaweizhenke-tang)
Reference
1
1. Frigas E, Loegering DA, Solley GO, Farrow GM, Gleich GJ. Elevated levels of the eosin- ophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clinic Proceedings. 1981;56:345-53.
2
2. 楊維傑 編. 黃帝內經譯解(素問). 서울:成輔社. 1980:72, 239.
3
3. 李圭晙. 素門大要. 서울:大成醫學社. 1999:32.
4
4. 許浚. 東醫寶鑑. 서울:驪江出版社. 1994:1827.
5
5. 金聖勳, 金東熙, 金基石, 宋昊哲 編著. 東醫病理學. 大田:周珉出版社. 1999:311, 411.
6
6. 강정수. 한의학개론. 대전:대전대학교 한의과대학. 1995:85, 174.
7
7. 黃泰康 總編, 王鵬 等 主編. 中醫哮病學. 北京:中國醫藥科技出版社. 2002:5-7, 9, 48-52.
8
8. 朱震亨 著, 대표역자 장일무, 지제근. 丹溪醫集. 서울:서울대학교 출판부. 2004:261.
9
9. 정영재. 大靑龍湯 등 數種의 處方이 기도배상세포에서의 mucin분비에 미치는 영향에 관한 연구. 경주:동국대학교 대학원 박사학위 논문. 2006.
10
10. 김호. 小靑龍湯加石膏 및 《石室秘籙》小治法方이 호흡기 점액의 분비에 미치는 영향. 경주:동국대학교 대학원 석사학위 논문. 2006.
11
11. 박완열. 大靑龍湯 및 《石室秘籙》急治法方이 호흡기 점액의 분비에 미치는 영향. 경주:동국대학교 대학원 석사학위 논문. 2006.
12
12. Kim KC, Opaskar-Hincman H, Bhaskar KR. Secretions from primary hamster tracheal surface epithelial cells in culture. Experimental Lung Research. 1989;15:299-314.
13
13. Ko KH, Lee CJ, Shin CY, Jo MJ, Kim KC. Inhibition of mucin release from airway goblet cells by polycationic peptides. American Journal of Physiology. 1999;277(21):811-5.
14
14. Kim KC. Possible requirement of collagen gel substratum for production of mucinlike glyco- proteins by primary rabbit tracheal epithelial cells in culture. In Vitro. 1985;21:617-21.
15
15. Kim KC, Rearick JI, Nettesheim P, Jetten AM. Biochemical characterization of mucous glyc- oproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. Journal of Biological Chemistry. 1985;260: 4021-7.
16
16. Kim KC, Brody JS. Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel. The Journal of Cell Biology. 1987;105:158a.
17
17. Wu R, Smith D. Continuous multiplication of rabbit tracheal epithelial cells in a defined, hormone-supplemented medium. In Vitro. 1982; 18:800-12.
18
18. Wu R, Nolan E, Turner C. Expression of tracheal differentiated function in serum-free hormone-supplemented medium. Journal of Cell Physiology. 1985;125:167-81.
19
19. Lee CJ. Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides. Journal of Applied Pharmacology. 2001;9(3):218-23.
20
20. Ko KH, Lee CJ, Shin CY, Jo MJ, Kim KC. Inhibition of mucin release from airway goblet cells by polycationic peptides. American Journal of Physiology. 1999;277(21):811-5.
21
21. 이충재. 설치류 기관 뮤신유리 억제에서의 폴리양이온성 작용기전. 서울:서울대학교 대학원 박사학위 논문; 1997.
22
22. Cheng PW, Sherman JM, Boat TE, Bruce M. Quantitation of radiolabeled mucous glycoprt- eins secreted by tracheal explants. Analytical Biochemistry. 1981;117:301-6.
23
23. Kim YD, Kwon EJ, Park DW, Song SY, Yoon SK, Baek SH. Interleukin-1beta induces MUC2 and MUC5AC synthesis through cyclooxyge- nase-2 in NCI-H292 cells. Molecular Pharm- acology. 2002;62(5):1112-8.
24
24. Karlinsey J, Stamatoyannopoulos G, Enver T. Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells. Anal- ytical Biochemistry. 1989;180(2):303-6.
25
25. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New Colorimetric Cytotoxicity Assay for Anticancer Drug Scre- ening. Journal of National Cancer Institute. 1990;82(13):1107-12.
26
26. 이정은, 박양춘. 淸金降火湯및 瓜蔞枳實湯이 呼吸器杯狀細胞로부터의 mucin 分泌에 미치는 영향. 대한한방내과학회지. 2004;6(2):242-3.
27
27. Newhouse MT, Biennenstock J. Respiratory tract defense mechanism. In: textbook of pul- monary disease(Baum GL, Wolinsky E(eds). 3rd edition. Boston:Little Brown and Company. 1983:2-47.
28
28. Naziruddin B, Shankar V, Reyes de la Rocha S, Sachdev GP. Polymeric structure of human respiratory mucin. Biochimica et biophysica acta. 1990;1041(2):164-71.
29
29. 이충재. 호흡기 배상세포에서 폴리양이온성 펩티드에 의해 야기되는 mucin유리 억제 현상의 특이성 규명. 응용약물학회지. 2001;9(3): 216-23.(489)
30
30. Cross CE, van der Vliet A, O'Neill CA, Louie S, Halliwell B. Oxidants, antioxidants, and respiratory tract lining fluids. Environ Health Perspect. 1994;102(10):185-91.
31
31. Kim KC, Zheng QX, Van-Seuningen I. Invol- vement of a signal transduction mechanism in ATP-induced mucin release from cultured airway goblet cells. American journal of respiratory cell and molecular biology. 1993; 8(2):121-5.
32
32. Mutschler E, Derendorf H. Drug actions. Boca Raton, Florida:CRC press Inc. 1995:410-1.
33
33. 張介賓. 景岳全書. 台北:台聯國風出版社. 1972: 986.
34
34. 李鍾馨. 晴崗醫鑑. 서울:成輔社. 1984:103.
35
35. 李珩九. 東醫肺系內科學. 서울:民瑞出版社. 1984: 18.
36
36. 申載鏞. 方藥合編解說. 서울:傳統醫學硏究所. 1989:51.
37
37. 韓醫科大學 方劑學敎室 共編著. 方劑學. 서울:永林社. 1992:304.
38
38. 上海中醫學院. 中醫內科學. 香港:商務印書館. 1982:24-5.
39
39. Freshney RI. Measurement of viability and cytotoxicity in culture of animal cells. 3rd edn. New York:Willey-Liss Inc. 1994:288.
40
40. Yu XY, Schofield BH, Croxton T, Takahashi N, Gabrielson EW, Spannhake EW. Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure. American journal of respiratory cell and molecular biology. 1994;11(2):188-98.
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