- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.3, pp.50-62
&
(2008). Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model. Journal of Korean Medicine, 29(3), 50-62.
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Abstract
Objectives:This study was aimed to find the effect of Samiunkyungtang on inflammation and microflora in an ulcerative colitis animal model. Methods:We established four groups of normal, control, test 1, and test 2 and assigned 6 rats toeach group. The normal group was not treated by any process and fed by normal saline. The control & test groups were provided with 4% dextran sodium sulfate (DSS) treatment for 7 days. Samiunkyungtang extract was orally administered to test groups (test 1=25mg/kg, test 2 100mg/kg) 3 days after DSS treatment for 10 days. After DSS treatment finished, we sacrificed the mice and measured colon length and enzyme activities such as myeloperoxidase (MPO), alkine phosphatase (ALP), β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase. Results:The colon lengths of test 1 and 2 groups were longer than the control group (p<0.05). Histologically, the crypts and superficial epitheliums of test 1 and 2 groups were regenerated. Goblet cells from all test groups were retrieved. The inflammatory biochemical marker, MPO and ALP activities in all test groups were highly reduced (p<0.01) compared to the control group. The activities of fecal bacterial enzymes in test groups such as β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase were reduced compared to the control group (p<0.01). Conclusions:As a result of this experiment, Samiunkyungtang is considered to have an inhibitory effect on inflammation and fecal enzyme activity in DSS-induced colitis animal model. Our results indicate that Samiunkyungtang may possess therapeutic effect on the development of DSS-induced colitis.
- keywords
- Samiunkyungtang, ulcerative colitis, dextran sodium sulfate (DSS), fecal enzymes
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