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박윤아(서울여자대학교) ; 맹수현(서울여대) ; 스리니바산 사티야라지(서울여자대학교) pp.197-202 https://doi.org/10.12651/JSR.2023.12.3.197
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The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea

정현영() ; Pablo Quintela-Alonso(Complutense University of Madrid) ; 정재호(강릉원주대학교) pp.203-211 https://doi.org/10.12651/JSR.2023.12.3.203
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Five ciliate species of Euplotes were isolated from fresh and coastal water during a sampling survey to identify unrecorded ciliates in South Korea. Their morphology was investigated using live observation, protargol and “wet” silver nitrate staining methods. Brief descriptions and microphotographs of each species and a comparison with related species are provided. Euplotes focardii is characterized by an average size of 65×47 μm after protargol impregnation, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-eurystomus type. Euplotes nobilii shows an average size of 34×20 μm after protargol staining, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-patella type. Euplotes octocarinatus, the only freshwater species described in the present study, is characterized by an average size of 66×46 μm after protargol impregnation, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-patella type. Euplotes petzi has an average size of 43×30 μm after protargol staining, a macronucleus hook-shaped and dorsal argyrome pattern in double-patella type. Euplotes raikovi is characterized by an average size of 40×24 μm after protargol staining, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double patella type.

YUNJEONGLEE(중앙대학교) ; Jung-Hoon Yoon(Sungkyunkwan University) ; 김명겸(서울여자대학교) ; Kiseong Joh(Hankuk University of Foreign Studies) ; 김승범(충남대학교) ; 전체옥(중앙대학교) ; Chang-Jun Cha(Chung-Ang University) ; Wan-Taek Im(Hankyong National University) ; Won Yong Kim(Chung-Ang University) pp.212-223 https://doi.org/10.12651/JSR.2023.12.3.212
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A total of 30 bacterial strains were identified in the classes Betaproteobacteria and Gammaproteobacteria in the study of prokaryotic species in Korea. These strains were isolated from a variety of environmental sources, including soil, tidal flat, mud, wetland, pine cone, seaweed, sea sediment, and brackish water. Phylogenetic analysis showed that isolates were identified based on high 16S rRNA gene sequence similarities (≥98.7%) with the predefined bacterial type species. In this study, we present data on previously unreported species from Korea, including 10 species from three families of one order in the class Betaproteobacteria and 20 species from 12 families of nine order in the class Gammaproteobacteria. Morphological, biochemical characteristics, isolation sources, and NIBR deposit numbers are provided in the description sections.

Chang-Moon Jang(Incheon National University) ; 배양섭(인천대학교) ; 유정선(환경부) ; Sue Yeon Lee(건국대학교) ; 김승태(건국대학교 생명환경연구소) pp.224-228 https://doi.org/10.12651/JSR.2023.12.3.224
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Three wolf spiders of the family Lycosidae Sundevall, 1833 are newly described from Korea: Arctosa labiata Tso & Chen, 2004, Pardosa altitudis Tikader & Malhotra, 1980, and Pardosa laevitarsis Tanaka & Suwa, 1986. These spiders were collected during a seasonal survey of the spider fauna of National Parks located in Gangwon-do and Gyeongsanbuk-do in 2018-2019; two males of A. labiata from Mt. Songnisan National Park, one female of P. altitudis from Mt. Chiaksan National Park, and two males of P. laevitarsis from Mt. Odaesan and Sobaeksan National Parks. The three newly recorded spiders have previously been known to be distributed in Taiwan, India, China, and Japan. These spiders were collected by hand in mixed forest leaf litter in mountainous terrains. The present paper taxonomically describes these three wolf spiders with measurements and morphological illustrations.

김인협(동국대학교) ; Wan-Taek Im(국립한경대학교 농업생명과학대학 생명공학과) ; Kiseong Joh(Hankuk University of Foreign Studies) ; 김명겸(서울여자대학교) ; Jung-Hoon Yoon(Sungkyunkwan University) ; Won Yong Kim(Chung-Ang University) ; 서태건(동국대학교) pp.229-239 https://doi.org/10.12651/JSR.2023.12.3.229
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We isolated and identified 25 unrecorded bacterial species belonging to the phylum Actinomycetota found in the Republic of Korea. Sequence comparison of 16S rRNA was performed using the NCBI BLAST and EzBioCloud database to identify 25 species, which had a 16S rRNA gene sequence similarity of >98.8% and were allocated as unrecorded species in the Republic of Korea. Among the 25 unrecorded bacterial strains, Streptomyces was the most common with nine species, followed by Leifsonia with two species. Isoptericola, Nocardioides, Dermacoccus, Sinomonas, Patulibacter, Marmoricola, Allobranchiibius, Aldersonia, Actinokineospora, Agromyces, Aeromicrobium, Cellulomonas, and Gordonia with one species each were also found. Twenty-five unrecorded species were excavated in various environments, such as tidal flats, ferns, soil, pine cones, moss, mud, wetlands, and plants. These isolates were characterized on the basis of their phylogenetic, biochemical properties, and morphological data, and species descriptions were provided.

정상우(DASARI Research Institute of BioResources) ; 신이찬(국립공원연구원 생태조사부) ; 김윤호(다살이 생물자원연구소) pp.240-243 https://doi.org/10.12651/JSR.2023.12.3.240
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The genus and species, Diekeana insignis(Gorham), is reported for the first time in Korea. Male adults were collected from Geoje Island and Changwon-si, located in the southern part of South Korea. The species of D. insignis(Gorham) is characterized by the following morphological characteristics: body length about 9.4mm; pronotum with transverse black marking in the middle part; each elytron with seven large black markings; penis long, slightly bent at apical part, truncate at apex; parameres narrow and as long as penis guide; penis guide narrow and pointed at apex. We herein provide habitus photographs, illustrations of morphological characters, male genitalia, and a detailed diagnosis. A partial sequence of the mitochondrial COI gene was obtained and provided mtDNA information for this species.

방현우(목원대학교) pp.244-257 https://doi.org/10.12651/JSR.2023.12.3.244
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The family Ameiridae Boeck, 1865 (Copepoda; Harpacticoida) consists of about 380 species in 47 genera, but only 10 species have been reported in Korea. A benthic ameirid species was collected from the East Sea of Korea. Specimens were identified as members of the genus Ameiropsis Sars G.O., 1907, and this is the first record of the genus Ameiropsis in the Pacific Ocean. Ameiropsis gyeongjuensis sp. nov. is morphologically similar to A. mixta Sars G.O., 1907 and A. longicornis Sars G.O., 1907 from Norway. However, the new species is clearly distinguishable from the others based on the following morphological characteristics: the proximal segment of antennary exopod with a bulge in the middle, and with one pinnate seta; the distal segment of antennary exopod with a thickened, blade-shaped outer seta; and female P5 baseoendopod with five setae and exopod with six setae. An identification key to species is provided for the 11 valid species of the genus Ameiropsis.

Chikako Ishii() ; Satoshi Kakishima() ; 박찬호(국립생물자원관) ; Takuro Ito() ; Atsushi Abe() ; Goro Kokubugata(National Museum of Nature and Science) ; 송관필((사) 한라산생태문화연구소) pp.258-265 https://doi.org/10.12651/JSR.2023.12.3.258
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We conducted phylogenetic analyses using multiplexed inter-simple sequence repeat genotyping by sequencing and compared chloroplast DNA sequences among Ardisia japonica, A. pusilla, and morphologically intermediate plants found on Jeju Island, Korea. Our network analysis demonstrated that the intermediate plants were genetically positioned between A. japonica and A. pusilla. Our comparison of the intergenic spacer between the psbA and trnH genes in chloroplast DNA indicated that four nucleotide substitutions separate A. japonica and A. pusilla, whereas the intermediate plants exhibited the A. japonica haplotype. Our results suggest that the intermediate plants on Jeju Island represent a natural hybrid of A. japonica, as the maternal species, and A. pusilla, and that they are attributable to Ardisia×walkeri. This record constitutes the first documented occurrence of the hybrid taxon in Korea.

손재천(공주교육대학교) pp.266-266 https://doi.org/10.12651/JSR.2022.11.3.202
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Three species of Choreutidae, Choreutis atrosignata (Christoph, 1888), Choreutis japonica (Zeller, 1877), and Litobrenthia japonica (Issiki, 1930), are reported for the first time from Korea. The records of C. atrosignata and C. japonica are based on two females from Yeongwol, Gangwon Province and from the Island Chujado, Jeju Province, respectively. The latter species was obtained from two larvae collected on the leaves of Ficus erecta (Moraceae). Photographs and data of rearing the larvae are provided. The record of L. japonica was based on two specimens collected from the Islands, Bogildo and Wando, Jeollanam Province. The present data of L. japonica represent the first record of the subfamily Brenthiinae and the genus Litobrenthia from Korea. Distributions of two species C. japonica and L. japonica, previously known only from Japan, now extends to Korea. Habitus and genitalia of the species treated here are briefly described and illustrated, if possible. To sum up, the Korean Choreutidae comprises four genera and 10 species, nine in Choreutinae and one in Brenthiinae.

Journal of Species Research