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Journal of Wellbeing Management and Applied Psychology / Journal of Wellbeing Management and Applied Psychology, (E)2586-6036
2022, v.5 no.3, pp.1-5
https://doi.org/https://doi.org/10.13106/jwmap.2022.vol5.no3.1
LEE, Woo-Sik
KWON, Woo-Taeg

Abstract

Purpose: The purpose of this study is to investigate whether wetland has antimicrobial activity on pathogenic bacteria in the toilet bowl. Research design, data and methodology: Air-dried mud obtained from "Jilmoe Bog" wetland was packed and dissolved in the autoclaved saline. Antimicrobial susceptibility was assessed against three Gram-negative bacteria using disk diffusion method and broth dilution method. Identification of specific bacterium presented in wetland supernatant was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Incubation of three Gram-negative bacteria with wetland supernatant inhibited bacterial growth of the bacteria, otherwise increased prevalence of specific bacterium. It was confirmed that Pseudomonas putida was presented in wetland supernatant. Conclusions: The results presented in this study might provide the possibility to utilize wetland supernatant as a bioremediation of toilet bowl bacteria.

keywords
Antimicrobial susceptibility, Wetland, Disk diffusion method, Broth dilution method, Toilet hygiene

Journal of Wellbeing Management and Applied Psychology