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  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Effect of Microwave Irradiation Time on Microwave-Assisted Weak Acid Protein Hydrolysis

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.3, pp.79-83
https://doi.org/10.5478/MSL.2019.10.3.79
Dahee Kim (Chungnam National University)
Minhee Joo (Chungnam National University)
Dabin Lee (Chungnam National University)
Huu-Quang Nguyen (Chungnam National University)
Jeongkwon Kim (Chungnam National University)
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Abstract

Horse heart myoglobin (MYG) and bovine serum albumin (BSA) were hydrolyzed by microwave-assisted weak-acid hydrolysis for 10, 20, 30, 40, 50, and 60 min using 2% formic acid (FA) at 100°C. Generally, the number of identified peptides increased with increasing irradiation time, indicating that the duration of microwave irradiation is linked to the efficiency of hydrolysis. For MYG, irradiation for 60 min provided the highest number of identified peptides, the greatest sequence coverage values and the highest MASCOT score values among the investigated irradiation times. Irradiation of BSA for 50min, however, yielded a greater number of peptides than irradiation for 60 min due to the generation of miscleaved peptides after microwave irradiation for 50min.

keywords
weak acid hydrolysis, MALDI-MS, microwave, myoglobin, albumi


참고문헌

1

Sun, W. (2006). . Mol. Cell. Proteom, 5, 769-.

2

Inglis, A. S. (1983). . Methods Enzymol, 91, 324-.

3

Li, A. (2001). . Anal. Chem, 73, 5395-.

4

Hua, L. (2006). . Proteomics, 6, 586-.

5

서미영. (2013). Weak Acid Hydrolysis of Proteins. Bulletin of the Korean Chemical Society, 34(1), 27-28. http://dx.doi.org/10.5012/bkcs.2013.34.1.27.

6

남미현. (2018). Effects of Temperature and Acetonitrile on Microwave-Assisted Weak Acid Protein Hydrolysis. Mass Spectrometry Letters, 9(2), 46-50. http://dx.doi.org/10.5478/MSL.2018.9.2.46.

7

Ly, H. G. T. (2015). . Angew. Chem, 54, 7391-.

8

Shi, J.-H. (2016). . J. Photochem. Photobiol, 162, 14-.

9

Ghosh, S. (2015). . J. Colloid Interface Sci, 458, 9-.

투고일Submission Date
2019-06-17
수정일Revised Date
2019-07-06
게재확정일Accepted Date
2019-07-09
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Mass Spectrometry Letters