- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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Analysis of Entamoeba histolytica Membrane via LC-MALDI-TOF/TOF
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.3, pp.84-87
https://doi.org/10.5478/MSL.2019.10.3.84
(Universiti Sains Malaysia)
(Universiti Sains Malaysia)
(Universiti Sains Malaysia)
(Universiti Sains Malaysia)
(Universiti Sains Malaysia)
Ujang,
J. A., Noordin,
R., &
Othman,
N.
(2019). Analysis of Entamoeba histolytica Membrane via LC-MALDI-TOF/TOF. Mass Spectrometry Letters, 10(3), 84-87, https://doi.org/10.5478/MSL.2019.10.3.84
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Abstract
Liquid chromatography mass spectrometry is widely employed in proteomics studies. One of such instruments is the Liquid Chromatography (LC)-Matrix-assisted laser desorption ionisation (MALDI)-Time of flight (TOF) or LC-MALDI-TOF/TOF. In this study, this instrument was used to identify the membrane proteins of a protozoan parasite namely Entamoeba histo-lytica. It causes amoebiasis in human. The E. histolytica trophozoites were cultured prior to the membrane protein extraction using the conventional method, ProteoPrep® and ProteoExtract® kits. Then, the membrane protein extracts were tryptic- digested and analysed by LC-MALDI-TOF/TOF. Approximately, 194 proteins were identified and 27.8% (54) were predicted as membrane proteins having 1 to 15 transmembrane regions and signal peptides by combining all three extraction methods. Also, this study has discovered 3 unique proteins as compared to our previous study which merit further investigation.
- keywords
- LC-MALDI-TOF/TOF, protein identification, Entamoeba histolytica, membrane
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- Submission Date
- 2019-06-25
- Revised Date
- 2019-07-23
- Accepted Date
- 2019-07-23
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