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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
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  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development of Sensitive Analytical Method of Rhodanthpyrone A by a LC-MS/MS and its Application to Bioavailability Study in Rats

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.3, pp.88-92
https://doi.org/10.5478/MSL.2019.10.3.88
Bitna Kang (Dankook University)
Jeong A Yoon (Dankook University)
Song Im-Sook (Kyungpook National University)
Young Taek Han (Dankook University)
Min-Koo Choi (Dankook University)
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Abstract

A sensitive analytical method of rhodanthpyrone A in rat plasma was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rhodanthpyrone A and rhodanthpyrone B (internal standard) in rat plasma were extracted by a liq-uid-liquid extraction method with ethyl acetate. This extraction method gave results in high and reproducible extraction recovery in the range of 73.75-79.90% with no interfering peaks around the peak elution time of rhodanthpyrone A and B. The standard calibra- tion curves for rhodanthpyrone A ranged from 0.5 to 2000 ng/mL were linear with r 2 > 0.994 and the inter- and intra-day accuracy and precision and the stability were within acceptance criteria. Using this validated analytical method, pharmacokinetics of rhodanth-pyrone A following intravenous and oral administration of rhodanthpyrone A at doses of 2 mg/kg and 30 mg/kg, respectively, were investigated. Rhodanthpyrone A in rat plasma showed multi-exponential elimination pattern with high clearance and volume of dis-tribution values. The absolute oral bioavailability of this compound was calculated as 3.7%. Collectively, the newly developed sensi-tive LC-MS/MS analytical method of rhodanthpyrone A could be successfully applied to investigate the pharmacokinetic properties of this compound and would be useful for the further studies on the efficacy, toxicity, and biopharmaceutics of rhodanthpyrone A

keywords
Rhodanthpyrone A, LC-MS/MS, bioavailability, rat plasma


참고문헌

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투고일Submission Date
2019-06-18
수정일Revised Date
2019-08-05
게재확정일Accepted Date
2019-08-08
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Mass Spectrometry Letters