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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Lipid N-formylation Occurs During Fixation with Formalin

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.2, pp.35-40
Kim Min Jung (Kyung Hee University)
Lim Heejin (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Kim Muwoong (Kyung Hee University)
Choi Yuri (Kyung Hee University)
Nguyen Thy N. C. (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Park Seung Cheol (Kyung Hee University)
Kim Kwang Pyo (Kyung Hee University)
Jung Junyang (Kyung Hee University)
Kim Min-Sik (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
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Human tissues and organs can be preserved intact by fixation with formalin for the future analysis of biomolecules of interest. With the advances in high-throughput methods, numerous protocols have been developed and optimized to attain the most pathophysiological information out of biomolecules, including RNA and proteins, in formalin-fixed samples. However, there is no systematic study to examine the effects of formalin fixation on the lipidome of biological samples in a global fashion. In this study, we conducted a mass spectrometry-based analysis to survey the alteration in the lipidome of mice brains by fixation methods. A total of 308 lipids were quantitatively measured using triple quadrupole mass spectrometry. We found that most were unchanged after formalin fixation except for a few lipid classes such as phosphatidylethanolamine.

formalin fixation, N-formylation, mass spectrometry-based lipidomics

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Mass Spectrometry Letters