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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Lipid N-formylation Occurs During Fixation with Formalin

Lipid N-formylation Occurs During Fixation with Formalin

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.2, pp.35-40
https://doi.org/10.5478/MSL.2022.13.2.35
Min Jung Kim (Kyung Hee University)
Heejin Lim (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Muwoong Kim (Kyung Hee University)
Yuri Choi (Kyung Hee University)
Thy N. C. Nguyen (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Seung Cheol Park (Kyung Hee University)
Kwang Pyo Kim (Kyung Hee University)
Junyang Jung (Kyung Hee University)
Min-Sik Kim (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
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Abstract

Human tissues and organs can be preserved intact by fixation with formalin for the future analysis of biomolecules of interest. With the advances in high-throughput methods, numerous protocols have been developed and optimized to attain the most pathophysiological information out of biomolecules, including RNA and proteins, in formalin-fixed samples. However, there is no systematic study to examine the effects of formalin fixation on the lipidome of biological samples in a global fashion. In this study, we conducted a mass spectrometry-based analysis to survey the alteration in the lipidome of mice brains by fixation methods. A total of 308 lipids were quantitatively measured using triple quadrupole mass spectrometry. We found that most were unchanged after formalin fixation except for a few lipid classes such as phosphatidylethanolamine.

keywords
formalin fixation, N-formylation, mass spectrometry-based lipidomics


투고일Submission Date
2022-03-31
수정일Revised Date
2022-04-17
게재확정일Accepted Date
2022-04-19
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Mass Spectrometry Letters