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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Combining the Power of Advanced Proteome-wide Sample Preparation Methods and Mass Spectrometry for defining the RNA-Protein Interactions

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.4, pp.115-124
Liu Tong (China Academy of Chinese Medical Sciences)
Xia Chaoshuang (Northwest University)
Li Xianyu (China Academy of Chinese Medical Sciences)
Yang Hongjun (China Academy of Chinese Medical Sciences)
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Emerging evidence has shown that RNA-binding proteins (RBPs) dynamically regulate all aspects of RNA in cells and involve in major biological processes of RNA, including splicing, modification, transport, transcription and degradation. RBPs, as powerful and versatile regulatory molecule, are essential to maintain cellular homeostasis. Perturbation of RNA-pro- tein interactions and aberration of RBPs function is associated with diverse diseases, such as cancer, autoimmune disease, and neurological disorders. Therefore, it is crucial to systematically investigate the RNA-binding proteome for understanding inter- actions of RNA with proteins. Thanks to the development of the mass spectrometry, a variety of proteome-wide methods have been explored to define comprehensively RNA-protein interactions in recent years and thereby contributed to speeding up the study of RNA biology. In this review, we systematically described these methods and summarized the advantages and disadvan- tages of each method.

proteomics, mass spectrometry, RNA-binding proteins, enrichment, RNA-protein interactions

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