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  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

The Advanced Analytical Method Through the Quantitative Comparative Study of Taurine in Feed Using LC-MS/MS

The Advanced Analytical Method Through the Quantitative Comparative Study of Taurine in Feed Using LC-MS/MS

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.4, pp.133-138
https://doi.org/10.5478/MSL.2022.13.4.133
Yeong Jun Seon (National Agricultural Products Quality Management Service)
Hyung Ju Seo (National Agricultural Products Quality Management Service)
Jiye Yoon (National Agricultural Products Quality Management Service)
Hyunjeong Cho (National Agricultural Products Quality Management Service)
Sunghie Hong (National Agricultural Products Quality Management Service)
Seung Hwa Lee (National Agricultural Products Quality Management Service)
Tae Woong Na (National Agricultural Products Quality Management Service)
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초록

Taurine is a type of sulfur-containing amino acid having a sulfate functional group, that is biosynthesized from cyste- ine. It is mainly distributed in high concentrations in animal tissues and is known to have various effects such as osmotic pres- sure control, calcium control, anti-inflammatory, antioxidant, and hepatocellular protection. Also, taurine deficiency causes a variety of symptoms, including visual impairment. In particular, in the case of cats, taurine is not biosynthesized and must be supplied through food, so it is classified as an essential amino acid. In this study, an analysis method using mass spectrometry was developed instead of the commonly used derivatization method to quickly, environmentally, and precisely analyze taurine in various animal feeds. The developed analytical method showed good linearity (R 2 > 0.99), accuracy (81.97-105.78%), and pre- cision (0.07-12.37%). In addition, the developed method was further verified through quantitative comparison with the derivat- ization method. This developed method was used in the determination of taurine in 20 animal feed samples obtained from South Korea. The levels of taurine found ranged from 81.53 to 6,743.53 mg/kg. The developed analysis method will be used for the detection and quantification of taurine in domestic feed.

keywords
taurine, feed, LC-MS/MS, amino acid analyzer, comparative study

Abstract

Taurine is a type of sulfur-containing amino acid having a sulfate functional group, that is biosynthesized from cyste- ine. It is mainly distributed in high concentrations in animal tissues and is known to have various effects such as osmotic pres- sure control, calcium control, anti-inflammatory, antioxidant, and hepatocellular protection. Also, taurine deficiency causes a variety of symptoms, including visual impairment. In particular, in the case of cats, taurine is not biosynthesized and must be supplied through food, so it is classified as an essential amino acid. In this study, an analysis method using mass spectrometry was developed instead of the commonly used derivatization method to quickly, environmentally, and precisely analyze taurine in various animal feeds. The developed analytical method showed good linearity (R 2 > 0.99), accuracy (81.97-105.78%), and pre- cision (0.07-12.37%). In addition, the developed method was further verified through quantitative comparison with the derivat- ization method. This developed method was used in the determination of taurine in 20 animal feed samples obtained from South Korea. The levels of taurine found ranged from 81.53 to 6,743.53 mg/kg. The developed analysis method will be used for the detection and quantification of taurine in domestic feed.

keywords
taurine, feed, LC-MS/MS, amino acid analyzer, comparative study


투고일Submission Date
2022-10-08
수정일Revised Date
2022-11-07
게재확정일Accepted Date
2022-11-21
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