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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Organic Acid Profiling Analysis in Culture Media of Lactic Acid Bacteria by Gas Chromatography-Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.3, pp.74-77
https://doi.org/10.5478/MSL.2012.3.3.74
Jae Yeon Lee (Biotopia Co., Ltd.)
Duc-Toan Nguyen (Ajou University)
Young Shik Park (Biotopia Co., Ltd.)
Kyo Yeol Hwang (Biotopia Co., Ltd.)
Yong Seok Cho (Biotopia Co., Ltd.)
Kyung-Don Kang (Biotopia Co., Ltd.)
Jaehwan Yoon (Ajou University)
Jundong Yu (Korea Racing Authority)
Sung-Tae Yee (Sunchon National University)
Young Hwan Ahn (Ajou University)
Gwang Lee (Ajou University)
Su-Il Seong (Biotopia Co., Ltd)
Man-Jeong Paik (Sunchon National University)
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Abstract

Organic acid (OA) profiling analysis was performed in culture media from Lactobacillus pentosus K34 (L. pentosusK34) and Pediococcus lolli PL24 (P. lolli PL24) by gas chromatography-mass spectrometry (GC-MS) following methoxime/tert-butyldimethylsilyl derivatives. 12 OAs were positively identified in culture media. Most of OA levels from L. pentosus K34of hetero lactic fermentation were found to be higher when compared with those from P. lolli PL24 of homo lactic fermentation,which may explain different OA metabolism in each strain. In addition, the distorted dodecagonal star patterns were readily distinguishable,and the characteristics of each strain were well represented. The present study demonstrates that the OA metabolicprofiling method by GC-MS combined with star pattern recognition is useful for the monitoring study of characteristic OAmetabolism in various microorganisms.

keywords
Organic acid, Profiling analysis, Lactobacillus pentosus K34, Pediococcus lolli PL24, GC-MS


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투고일Submission Date
2012-05-30
수정일Revised Date
2012-06-29
게재확정일Accepted Date
2012-08-20
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