- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.3, pp.82-85
https://doi.org/10.5478/MSL.2012.3.3.82
(Sogang University)
(Sogang University)
(Korea Research Institute of Standards and Science)
(Korea Research Institute of Standards and Science)
(Sogang University)
(Sogang University)
(Sogang University)
(Korea Research Institute of Standards and Science)
(Korea Research Institute of Standards and Science)
(Sogang University)
(Sogang University)
Park,
S., Park,
S., Oh,
J. Y., Han,
S. Y., Jo,
K., &
Oh,
H.
B.
(2012). Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry. Mass Spectrometry Letters, 3(3), 82-85, https://doi.org/10.5478/MSL.2012.3.3.82
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Abstract
The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) wasexamined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc fingerpeptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn2+ in the native condition, electrospray of apo-zinc fingerin solution containing ZnCl2 or Zn(CH3COO)2 resulted in the formation of zinc finger−Zn2+ complexes with multiple zincions. This result suggests the formation of nonspecific zinc finger−Zn2+ complexes. Zn(tartrate), Zn(OOC(CHOH)2COO),mainly produced specific zinc finger−Zn2+ complexes with a single zinc ion. This study clearly indicates that tartrate is an excellentcounter ion in ESI-MS studies of zinc finger−Zn2+ complexes, which prevents the formation of nonspecific zinc finger−Zn2+complexes.
- keywords
- Zinc finger, Zinc ion, Electrospray ionization, Tartrate, Noncovalent complexes
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- Submission Date
- 2012-08-31
- Revised Date
- 2012-09-17
- Accepted Date
- 2012-09-17
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