ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.3, pp.82-85
https://doi.org/10.5478/MSL.2012.3.3.82
Soojin Park (Sogang University)
Sunhee Park (Sogang University)
Joo Yeon Oh (Korea Research Institute of Standards and Science)
Sang Yun Han (Korea Research Institute of Standards and Science)
Kyubong Jo (Sogang University)
Han Bin Oh (Sogang University)
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Abstract

The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) wasexamined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc fingerpeptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn2+ in the native condition, electrospray of apo-zinc fingerin solution containing ZnCl2 or Zn(CH3COO)2 resulted in the formation of zinc finger−Zn2+ complexes with multiple zincions. This result suggests the formation of nonspecific zinc finger−Zn2+ complexes. Zn(tartrate), Zn(OOC(CHOH)2COO),mainly produced specific zinc finger−Zn2+ complexes with a single zinc ion. This study clearly indicates that tartrate is an excellentcounter ion in ESI-MS studies of zinc finger−Zn2+ complexes, which prevents the formation of nonspecific zinc finger−Zn2+complexes.

keywords
Zinc finger, Zinc ion, Electrospray ionization, Tartrate, Noncovalent complexes


참고문헌

1

Fenn, J. B. (1989). . Science, 246, 64-.

2

Katta, V. (1991). . J. Am. Chem. Soc, 113, 8534-.

3

Lim, H. K. (1995). . J. Mass Spectrom, 30, 708-.

4

Cheng, X. (1995). . J. Am. Chem. Soc, 117, 8859-.

5

Loo, J. A. (1997). . Mass Spectrom. Rev, 16, 1-.

6

Rostom, A. A. (1999). . Curr. Opin. Struct. Biol, 9, 135-.

7

Oh, H. B. (2002). . Proc. Nat’l. Acad. Sci. U.S.A, 99, 15863-.

8

Lee, S. Y. (2009). . Int. J. Mass Spectrom, 279, 47-.

9

Yeonmi Seong. (2011). An anomalous dissociation of protonated cluster ions of DNA guanine-cytosine base-pair. Mass Spectrometry Letters, 2(3), 73-75.

10

Hutchens, T. W. (1992). . Febs Lett, 309, 170-.

11

Hu, P. F. (1994). . Anal. Chem, 66, 4190-.

12

Feng, R. (1995). . J. Am. Soc. Mass Spectrom, 6, 1105-.

13

Cheng, X. (1995). . J. Am. Chem. Soc, 117, 8859-.

14

Rostom, A. A. (2000). . J. Mol. Biol, 296, 269-.

15

Kempen, E. C. (2000). . Anal. Chem, 72, 5411-.

16

Wang, W. (2003). . Anal. Chem, 75, 4945-.

17

Gabelica, V. (2003). . J. Mass Spectrom, 38, 491-.

18

Speir, J. P. (1995). . J. Mass Spectrom, 30, 39-.

19

Toli , L. P. (1998). . Anal. Chem, 70, 405-.

20

Yi, S. (2007). . Biochemistry, 46, 13120-.

21

Niessen, W. M. (1999). . J. Chromatogr. A, 856, 179-.

22

Cavanagh, J. (2003). . Anal. Chem, 75, 3281-.

23

Pan, J. (2009). . Anal. Chem, 81, 5008-.

24

Miller, J. (1985). . EMBO J, 4, 1609-.

25

Jamieson, A. C. (2003). . Nature Reviews Drug Discovery, 2, 361-.

26

Park, S. J. (2011). . Analyst, 136, 3739-.

27

Kriwacki, R. W. (1992). . Proc. Nat’l. Acad. Sci. U.S.A, 89, 9759-.

28

Hsu, T. (1992). . Science, 257, 1946-.

29

Gogos, J. A. (1996). . Proc. Nat’l. Acad. Sci. U.S.A, 93, 2159-.

투고일Submission Date
2012-08-31
수정일Revised Date
2012-09-17
게재확정일Accepted Date
2012-09-17
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