• P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.2, pp.38-40
https://doi.org/10.5478/MSL.2013.4.1.38
Kim Jeongkwon (Chungnam National University)
Kim Taehee (Chungnam National University)
Lee Jihyeon (Chungnam National University)
Im Haeju (Chungnam National University)
Kim Jeongkwon (Chungnam National University)
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Abstract

Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-offlight(MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycanpeaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.

keywords
Glycans, MALDI, PNGase F, ovalbumin, oligosaccharides, dihydroxybenzoic acid


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Submission Date
2013-06-23
Revised Date
2013-06-26
Accepted Date
2013-06-27
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