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- P-ISSN 2233-4203
- E-ISSN 2093-8950
Dioscin is a biologically active steroidal saponin with anticancer and hepatoprotective effects. A rapid, selective, andsensitive liquid chromatographic method with electrospray ionization tandem mass spectrometry was developed for the quantificationof dioscin in rat plasma. Dioscin was extracted from rat plasma using ethyl acetate at acidic pH. The analytes were separatedon a Halo C18 column using gradient elution of acetonitrile and 0.1% formic acid and detected by tandem massspectrometry in selected reaction monitoring mode. The standard curve was linear (r2 = 0.998) over the concentration range of1−100 ng/mL. The lower limit of quantification was 1.0 ng/mL using 50 μL of plasma sample. The coefficient of variation andrelative error for intra- and inter-assay at four QC levels were 1.3 to 8.0% and −5.4 to 10.0%, respectively. This method wasapplied successfully to the pharmacokinetic study of dioscin after oral administration of dioscin at a dose of 29.2 mg/kg in maleSprague-Dawley rats.
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