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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Liquid Chromatography-tandem Mass Spectrometry for Quantification of Dioscin in Rat Plasma

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.3, pp.55-58
https://doi.org/10.5478/MSL.2013.4.3.55
Tae Yeon Kong (The Catholic University of Korea)
Hye Young Ji (The Catholic University of Korea)
Sang-Zin Choi (Donga ST Co.)
Miwon Son (Donga ST Co.)
Hye Suk Lee (The Catholic University of Korea)
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Abstract

Dioscin is a biologically active steroidal saponin with anticancer and hepatoprotective effects. A rapid, selective, andsensitive liquid chromatographic method with electrospray ionization tandem mass spectrometry was developed for the quantificationof dioscin in rat plasma. Dioscin was extracted from rat plasma using ethyl acetate at acidic pH. The analytes were separatedon a Halo C18 column using gradient elution of acetonitrile and 0.1% formic acid and detected by tandem massspectrometry in selected reaction monitoring mode. The standard curve was linear (r2 = 0.998) over the concentration range of1−100 ng/mL. The lower limit of quantification was 1.0 ng/mL using 50 μL of plasma sample. The coefficient of variation andrelative error for intra- and inter-assay at four QC levels were 1.3 to 8.0% and −5.4 to 10.0%, respectively. This method wasapplied successfully to the pharmacokinetic study of dioscin after oral administration of dioscin at a dose of 29.2 mg/kg in maleSprague-Dawley rats.

keywords
Dioscin, LC-MS/MS, Rat plasma


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투고일Submission Date
2013-09-16
수정일Revised Date
2013-09-25
게재확정일Accepted Date
2013-09-25
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Mass Spectrometry Letters