- P-ISSN 2233-4203
- E-ISSN 2093-8950
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- P-ISSN2233-4203
- E-ISSN2093-8950
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Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2015, v.6 no.1, pp.7-12
https://doi.org/10.5478/MSL.2015.6.1.7
(Sogang University, Ministry of National Defense)
(Sogang University)
(Dongguk University)
(Sungkyunkwan University)
(Sogang University)
(Sogang University)
(Sogang University)
(Dongguk University)
(Sungkyunkwan University)
(Sogang University)
(Sogang University)
Lee,
C. S.,
Park,
S. j.,
Lee,
J. Y.,
Park,
S.,
Jo,
K.,
&
Oh,
H.
B.
(2015). . Mass Spectrometry Letters, 6(1), 7-12, https://doi.org/10.5478/MSL.2015.6.1.7
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Abstract
In the present study, we proposed a simple ESI-MS model for determining Zn2+ binding (or dissociation) constants forzinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α andβ, could be determined for ZiCo ZFP with a known Zn2+ binding constant. We could determine the binding constants for other ZFPsassuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constantsexhibited Kd values of 10-7~10-9 M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6 M and4.3×10-4 M for BBA1 and melittin, respectively.
- keywords
- zinc finger, binding constant, electrospray-mass spectrometry, zinc ion
참고문헌
1
Kudrin, A. V.. (2002). . J. Trace Elements in Experimental Medicine, 15, 103-.
2
Miller, J.. (1985). . EMBO J, , 1609-.
3
Hori, Y.. (2000). . J. Am. Chem. Soc, 12, 7648-.
4
Klug, A.. (1995). . FASEB J., 9, 597-.
5
Berg, J. M.. (1996). . Science, 271, 1081-.
6
Miura, T.. (1998). . Biochimica et Biophysica Acta, 1384, 171-.
7
Papworth, M.. (2006). . Gene, 366, 27-.
8
Briand, G.. (1997). . Arch. Biochem. Biophys, 339, 291-.
9
Loo, J. A.. (1997). . Mass Spectrom. Rev, 16, 1-.
10
Whittal, R. M.. (2000). . Proein Sci, 9, 395-.
11
Gehrig, P. M.. (2000). . Protein. Sci, 9, 395-.
12
Kramer, M. L.. (2001). . J. Biol. Chem, 276, 16711-.
13
Beck, J. L.. (2001). . Mass Spectrom. Rev, 20, 61-.
14
van den Bremer, E. T. J.. (2002). . Protein Sci, 11, 1738-.
15
Urvoas, A.. (2003). . Rapid Commun. Mass Spectrom, 17, 1889-.
16
Sudhir, P. P.. (2005). . Rapid Commun. Mass Spectrom, 19, 1517-.
17
Vriendt, K. D.. (2006). . J. Am. Soc. Mass Spectrom, 17, 180-.
18
Fabris, D.. (1999). . Inorg. Chem, 38, 1322-.
19
Fukuda, H.. (1999). . Bioorg. Med. Chem, 7, 1213-.
20
Hathout, Y.. (2001). . Int. J. Mass Spectrom, 204, 1-.
21
Loo, J. A.. (2001). . Int. J. Mass Spectrom, 204, 113-.
22
Larabee, J.. (2005). . Chem. Res. Toxicol, 18, 1943-.
23
Park, S. J.. (2011). . Analyst, 136, 3739-.
24
Soojin Park. (2012). Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry. Mass Spectrometry Letters, 3(3), 82-85. http://dx.doi.org/10.5478/MSL.2012.3.3.82.
25
Loo, J. A.. (1997). . J. Am. Soc. Mass Spectrom, 8, 234-.
26
Young, D. S.. (1997). . Rapid Commun. Mass Specrom, 11, 769-.
27
Przybylski, M.. (1996). . Angew. Chem. Int. Ed. Engl., 35, 806-.
28
S. Blair,. (1998). . J. Am. Soc. Mass Spectrom, 9, 1049-.
29
Kempen, E. C.. (2000). . Anal. Chem, 72, 5411-.
30
Wortmann, A.. (2005). . J. Mass Spectrom, 40, 777-.
31
Schmidt, A. C.. (2010). . Mass. Spectrom, 45, 870-.
32
Liu, J.. (2011). . J. Am. Soc. Mass. Spectrom, 22, 408-.
33
Cubrilovic, D.. (2014). . ACS Chem. Biol, 9, 218-.
34
Lee, S. S.. (2014). . Phys. Chem. Chem. Phys, 16, 8376-.
35
Cerasoli, E.. (2005). . J. Am. Chem. Soc, 127, 15008-.
36
Berg, J. M.. (1989). . J. Am. Chem. Soc, 111, 3759-.
37
Gabelica, V.. (2003). . J. Mass Spectrom, 38, 491-.
- 투고일Submission Date
- 2015-01-23
- 수정일Revised Date
- 2015-03-03
- 게재확정일Accepted Date
- 2015-03-04
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