ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2015, v.6 no.1, pp.7-12
https://doi.org/10.5478/MSL.2015.6.1.7
Choong Sik Lee (Sogang University, Ministry of National Defense)
Soo jin Park (Sogang University)
Jae Young Lee (Dongguk University)
Sungsu Park (Sungkyunkwan University)
Kyubong Jo (Sogang University)
Han Bin Oh (Sogang University)
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Abstract

In the present study, we proposed a simple ESI-MS model for determining Zn2+ binding (or dissociation) constants forzinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α andβ, could be determined for ZiCo ZFP with a known Zn2+ binding constant. We could determine the binding constants for other ZFPsassuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constantsexhibited Kd values of 10-7~10-9 M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6 M and4.3×10-4 M for BBA1 and melittin, respectively.

keywords
zinc finger, binding constant, electrospray-mass spectrometry, zinc ion


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투고일Submission Date
2015-01-23
수정일Revised Date
2015-03-03
게재확정일Accepted Date
2015-03-04
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