- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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LC-MS/MS Method for Simultaneous Analysis of Growth Hormone-Releasing Peptides and Secretagogues in Human Urine
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.3, pp.55-63
https://doi.org/10.5478/MSL.2016.7.3.55
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
Min,
H., Han,
B., Sung,
C., Park,
J., Lee,
K. M., Kim,
H. J., Kim,
K. H., Son,
J., Oh-SeungKwon,
, &
Jaeick,
L.
(2016). LC-MS/MS Method for Simultaneous Analysis of Growth Hormone-Releasing Peptides and Secretagogues in Human Urine. Mass Spectrometry Letters, 7(3), 55-63, https://doi.org/10.5478/MSL.2016.7.3.55
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Abstract
Growth hormone (GH)-releasing peptides (GHRPs) and GH secretagogues (GHSs) are listed in the World Anti-Doping Agency (WADA) Prohibited List. In the present study, we developed and validated a method for the simultaneous analysis of seven GHRPs (alexamorelin, GHRP-1, -2, -4, -5, -6, and hexarelin) and three GHSs (anamorelin, ibutamoren, and ipamorelin) in human urine. Method validation was performed at minimum required performance levels specified by WADA technical documents (2 ng/mL) for all substances, and the method was validated with regard to selectivity (no interference), linearity (R2 > 0.9986), matrix effects (50.0%-141.2%), recovery (10.4%-100.8%), and intra- (2.8%-16.5%) and inter-day (7.0%-22.6%) precisions. The limits of detection for screening and confirmation were 0.05-0.5 ng/mL and 0.05-1 ng/mL, respectively.
- keywords
- Doping control analysis, Growth hormone, GHRP, GHS, Method validation
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- Submission Date
- 2016-08-11
- Revised Date
- 2016-09-29
- Accepted Date
- 2016-09-29
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