- P-ISSN 2233-4203
- E-ISSN 2093-8950
- 권한신청
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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Mass Spectrometry in the Determination of Glycosylation Site and N-Glycan Structures of Human Placental Alkaline Phosphatase
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2018, v.9 no.3, pp.67-72
https://doi.org/10.5478/MSL.2018.9.3.67
(Erzincan University)
(Rensselaer Polytechnic Institute)
(Rensselaer Polytechnic Institute)
(Rensselaer Polytechnic Institute)
(Rensselaer Polytechnic Institute)
Solakyildirim,
K.,
Li,
L.,
&
RobertJ..
(2018). . Mass Spectrometry Letters, 9(3), 67-72, https://doi.org/10.5478/MSL.2018.9.3.67
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Abstract
Alkaline phosphatase (AP) is a membrane-bound glycoprotein that is widely distributed in the plasma membrane of cells of various organs and also found in many organisms from bacteria to humans. The complete amino acid sequence and three-dimensional structure of human placental alkaline phosphatase have been reported. Based on the literature data, AP con- sists of two presumptive glycosylation sites, at Asn-144 and Asn-271. However, it only contains a single occupied N-linked gly- cosylation site and no occupied O-linked glycosylation sites. Hydrophilic interaction chromatography (HILIC) has been primarily employed for the characterization of the glycan structures derived from glycoproteins. N-glycan structures from human placental alkaline phosphatase (PLAP) were investigated using HILIC-Orbitrap MS, and subsequent data processing and glycan assignment software. 16 structures including 10 sialylated N-glycans were identified from PLAP.
- keywords
- Alkaline phosphatase, glycosylation, N-glycans, Mass spectrometry, HILIC
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- 투고일Submission Date
- 2018-04-04
- 수정일Revised Date
- 2018-06-29
- 게재확정일Accepted Date
- 3028-05-30
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