ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

논문 상세

Home > 논문 상세
  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Characterization of Molecular Composition of Bacterial Melanin Isolated from Streptomyces glaucescens Using Ultra-High-Resolution FT-ICR Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2018, v.9 no.3, pp.81-85
https://doi.org/10.5478/MSL.2018.9.3.81
Mira Choi (Korea Basic Science Institute)
A Young Choi (Korea Basic Science Institute)
Soo-Yeon Ahn (Ajou University)
Kwon-Young Choi (Ajou University)
Kyoung-Soon Jang (Korea Basic Science Institute)
  • 다운로드 수
  • 조회수

Abstract

In this study, the chemical composition of bacterial melanin isolated from the Streptomyces glaucescens strain was elucidated by ultra-high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Ultra-high-resolu- tion mass profiles of the microbial melanin product were acquired using a 15 Tesla FT-ICR mass spectrometer in positive and negative ion modes via electrospray ionization to obtain more complete descriptions of the molecular compositions of melanin- derived organic constituents. A mass resolving power of 500,000 (at m/z 400) was achieved for all spectra while collecting 400 scans per sample with a 4 M transient. The results of this analysis revealed that the melanin pigment isolated from S. glaucescens predominantly exhibits CHON and CHO species, which belong to the proteins class of compounds, with the mean C/O and C/N ratios of 4.3 and 13.1, thus suggesting that the melanin could be eumelanin. This analytical approach could be utilized to investi- gate the molecular compositions of a variety of natural or synthetic melanins. The compositional features of melanins are important for understanding their formation mechanisms and physico-chemical properties.

keywords
Melanin, Streptomyces glaucescens, FT-ICR MS, Chemical composition, Eumelanin


참고문헌

1

Chedekel, M. R.. (1992). . Pigment Cell Res., 5, 240-. http://dx.doi.org/10.1111/j.1600-0749.1992.tb00543.x.

2

Solano, F.. (2014). . New J. Sci., 2014, 498276-.

3

Plonka, P. M.. (2006). . Acta Biochim. Pol., 53, 429-.

4

Nosanchuk, J. D.. (2006). . Antimicrob. Agents Chemother., 50, 3519-. http://dx.doi.org/10.1128/AAC.00545-06.

5

Brenner, M.. (2008). . Photochem. Photobiol., 84, 539-. http://dx.doi.org/10.1111/j.1751-1097.2007.00226.x.

6

Riley, P. A.. (1997). . Int. J. Biochem. Cell Biol., 29, 1235-. http://dx.doi.org/10.1016/S1357-2725(97)00013-7.

7

Jacobson, E. S.. (1993). . J. Bacteriol., 175, 7102-. http://dx.doi.org/10.1128/jb.175.21.7102-7104.1993.

8

El-Naggar, N. E.. (2017). . Sci. Rep., 7, 42129-. http://dx.doi.org/10.1038/srep42129.

9

Prados-Rosales, R.. (2015). . J. Agric. Food Chem., 63, 7326-. http://dx.doi.org/10.1021/acs.jafc.5b02713.

10

Antony, R.. (2014). . Environ. Sci. Technol., 48, 6151-. http://dx.doi.org/10.1021/es405246a.

11

Cho, Y.. (2015). . Mass Spectrom. Rev., 34, 248-. http://dx.doi.org/10.1002/mas.21438.

12

Guigue, J.. (2016). . Biogeochemistry, 128, 307-. http://dx.doi.org/10.1007/s10533-016-0209-5.

13

Ksionzek, K. B.. (2016). . Science, 354, 456-. http://dx.doi.org/10.1126/science.aaf7796.

14

Lobodin, V. V.. (2014). . Energy Fuels, 28, 6841-. http://dx.doi.org/10.1021/ef501683w.

15

Mazur, D. M.. (2016). . Sci. Total Environ., 557, 12-.

16

Marshall, A. G.. (2013). . Mass Spectrom. (Tokyo), 2, S0009-.

17

Kim, S.. (2003). . Anal. Chem., 75, 5336-. http://dx.doi.org/10.1021/ac034415p.

18

Wu, Z.. (2004). . Anal. Chem., 76, 2511-. http://dx.doi.org/10.1021/ac0355449.

19

Jeong, H. J.. (2018). . ACS Omega, 3, 7441-. http://dx.doi.org/10.1021/acsomega.8b00697.

20

Cha, J. Y.. (2017). . J. Agric. Food Chem., 65, 1167-. http://dx.doi.org/10.1021/acs.jafc.6b04700.

21

최정훈. (2017). Chemical Characterization of Dissolved Organic Matter in Moist Acidic Tussock Tundra Soil Using Ultra-high Resolution 15T FT-ICR Mass Spectrometry. Biotechnology and Bioprocess Engineering, 22(5), 637-646.

22

Choi, J. H.. (2017). . Environ. Pollut., 225, 329-. http://dx.doi.org/10.1016/j.envpol.2017.02.058.

23

Koch, B. P.. (2005). . Geochim. Cosmochim. Acta, 69, 3299-. http://dx.doi.org/10.1016/j.gca.2005.02.027.

투고일Submission Date
2018-08-14
수정일Revised Date
2018-09-01
게재확정일Accepted Date
2018-09-09
상단으로 이동

Mass Spectrometry Letters