- P-ISSN 1010-0695
- E-ISSN 2288-3339
Objectives: The water extract of Samul-Tang(SMT) has been traditionally used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SMT rescues cells from these damages. Methods: Therefore, this study was designed to investigate the protective mechanisms of SMT on oxidative stress-induced toxicity in H9c2 cardiomyoblast cells. Treatment of H2O2 markedly induced death of H9c2 cardiomyoblast cells in a dose-dependent manner. Results: The characteristics of H2O2-induced death of H9c2 showed apparent apoptotic features such as DNA fragmentation and morphological change. However, SMT significantly reduced both H2O2-induced cell death and morphological change. The decrease of Bcl-2 expression by H2O2 were inhibited by SMT. In addition, the increase of Bax expression was also inhibited by SMT. The cotreatment of SMT and H2O2 in H9c2 cells also induced the phosphorylation of ERK in a time-dependent manner. Moreover, PD98059, a specific inhibitor of ERK1/2 attenuated the protective effects of SMT on H2O2-induced toxicity in H9c2 cardiomyoblast cells. These results suggest that both ERK1/2 signaling pathways play important roles in the protective effects of SMT on H2O2-induced apoptotic death of H9c2 cells. Also, I have screened the expression profile of proteins in H9c2 cardiomyoblast cells by using two-dimensional (2-D) gel electrophoresis. Among 300 spots resolved in 2-D gels, the comparison of control versus apoptosis cells revealed that signal intensity of 17 spots increased and 11 spots decreased. Conclusions: Taken together, this study suggests that the protective effects of the water extract of SMT against oxidative damages may be mediated by the modulation of Bcl-2 and Bax expression via the regulation of ERK signaling pathway.
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