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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

A Study on the association between tumor necrosis factor alpha gene polymorphism and Sasang Constitution in cerebral infarction

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.1, pp.59-70










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Abstract

Objectives : Tumor necrosis factor-α (TNF-α), a potent immuno-modulator and pro-inflammatory cytokine, has been implicated in many pathological processes. In this study, the authorexamined whether promoter region polymorphism in the TNF-α gene at position –308 affect the odds of cerebral infarction (CI) and whether genetic risk is enhanced by Sasang constitutional classification. Methods : 212 CI patients and 610 healthy controls were genotyped and determined according to Sasang constitutional classification. The amplified genotypes were analyzed on 8% polyacrylamide gel. The alleles were visualized by ethidium bromide staining. Primers for TNF-α were designed to incorporate a polymorphic site at a position –308 bp of the TNF-α gene into an NcoI restriction site. Restriction digests generated products of 87 and 20 bp for G allele and 107 bp for A allele. Results : A significant decrease was found for the TNF-α A allele in CI patients compared with controls (P=0.033, odds ratio, O.R.: 0.622). However, there was no significant association between TNF-α polymorphism and Sasang constitution in CI patients. Conclusions : My finding suggests that TNF-α promoter region polymorphism is responsible for susceptibility to CI in Koreans.

keywords
tumor necrosis factor-α, TNF-α, Sasang constitution, cerebral infarction, genotype, Korean, tumor necrosis factor-α, TNF-α, Sasang constitution, cerebral infarction, genotype, Korean


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