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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Study on Relationship between Tumor Necrosis Factor-α Gene Polymorphism and Obese Patients

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.1, pp.85-92



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Abstract

Objectives: A number of candidate genes have been in implicated in the pathogenesis of obesity in humans. Tumor necrosis factor-alpha (TNFα) is expressed primarily in adipocytes, and elevated levels of this cytokine have been linked to obesity and insulin resistance. Recently, the A allele of a polymorphism at position 308 in the promoter region of TNFα (G-308A) has been shown to increase transcription of the gene in adipocytes. Therefore, we designed this study to test whether obese and non-obese subjects differ from TNFα genotype distribution, and the genotypes affect the anthropometric parameters, including the degrees of body mass index (BMI). Methods: The study included 153 obese healthy women (BMI≥25 kg/m2, range 25-54.7, age range 15-40 years), 82 non-obese women (BMI<25 kg/m2, age range 15-40 years). Total fat mass and percent body fat were determined by dual-energy X-ray absorptiometry. Genomic DNA was extracted and used for NcoI restriction fragment length polymorphism (RFLP) based genotyping of TNFα. Results: No differences were observed for allelic and genotype frequencies between obese women (BMI≥25) and 82 non-obese women. Also, no association of TNFα polymorphism was observed with body mass index (BMI) for genotype in obese women. In addition, age, percent body fat, BMI, and cholesterol levels did not differ from TNFα genotype. However, waist-to-hip ratio (WHR) was significantly decreased in subjects with TNFα GA or AA genotype (0.94 0.07 vs. 0.92 0.03, P<0.005). Conclusion: These results suggest that TNFα promoter polymorphism at position -308 is not a significant factor for BMI, but affects the WHR in obese healthy women from Koreans.

keywords
비만, TNF- α 유전자, 복부비만도 (WHR), Obesity, TNFα gene, Waist-to-hip ratio (WHR), Obesity, TNFα gene, Waist-to-hip ratio (WHR)


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