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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

The Effects of Platycodi Radix on The Induction of LPS and the Activation of NF-κBp, the Lung Disease of White Rats

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.1, pp.18-25



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Abstract

Objectives & Methods : We examined the effects of Platycodi Radix on the process of lipopolysaccharide (LPS)-induced nuclear factor (NF)-κBp65 and inhibitory (I)-κBα alteration in RAW 264.7 cell and acute lung injury in rats. Results : Immunoblot analysis showed that LPS-induced degradation of I-κBα in RAW 264.7 was inhibited by pretreatment of Platycodi Radix. The total cells of bronchoalveolar lavage fluid by LPS challenge markedly decreased in the Platycodi Radix pretreatment rats. Platycodi Radix pretreatment caused also a decline in neutrophils infiltration into interstitium of the lung. In the alveolar macrophages and neutrophils, decreased NF-κBp65 and inducible nitric oxide synthase and increased I-κBα immunoreaction were detected in Platycodi Radix pretreated rats compared with LPS alone treated ones.Conclusion : It may be concluded that Platycodi Radix attenuates the development of LPS-induced inflammation by reduction of NF-κBp65 activation and neutrophil-mediated acute lung injury. Platycodi Radix would be useful as a therapeutic agent for endotoxin-induced lung disease.

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