ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

논문 상세

Home > 논문 상세
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

The Effects of Platycodi Radix on The Induction of LPS and the Activation of NF-κBp, the Lung Disease of White Rats

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.1, pp.18-25
김현중 (동의대학교)
박동일 (동의대학교)
김원일 (동의대학교)
  • 다운로드 수
  • 조회수

초록

전통적으로 호흡기계 질환에 널리 쓰여져 온 길경이 LPS유발 염증에 미치는 효과를 알기 위하여 마우스 대식세포 RAW 264.7의 NF-κBp65와 I-κBα발현에 대한 Western blot, 흰쥐 폐조직에서 NF-κBp65, I-κBα 및 iNOS 발현을 면역조직화학적으로 살펴보았다.배양 대식세포에서 길경의 전처리는 LPS에 의한 I-κBα분해를 저해시켜 NF-κBp65 활성을 억제하였다. 흰쥐에 대한 길경 전처리는 기관지폐포세척액내 총세포수를 감소시킬 뿐 아니라 폐포강 내 관찰되는 염증세포와 폐중격 내 중성호성백혈구의 침적을 억제하였다. 그리고 폐포대식세포와 중성호성백혈구에서 NF-κBp65와 iNOS 발현을 감소시키며 I-κBα의 발현을 증가시켰다. 이상의 결과로 길경은 NF-κB를 중심으로 한 염증관련인자를 조절하여 중성호성 백혈구침적을 막음으로서 LPS 투여에 의한 폐손상을 완화시키는 것을 알 수 있다.

keywords

Abstract

Objectives & Methods : We examined the effects of Platycodi Radix on the process of lipopolysaccharide (LPS)-induced nuclear factor (NF)-κBp65 and inhibitory (I)-κBα alteration in RAW 264.7 cell and acute lung injury in rats. Results : Immunoblot analysis showed that LPS-induced degradation of I-κBα in RAW 264.7 was inhibited by pretreatment of Platycodi Radix. The total cells of bronchoalveolar lavage fluid by LPS challenge markedly decreased in the Platycodi Radix pretreatment rats. Platycodi Radix pretreatment caused also a decline in neutrophils infiltration into interstitium of the lung. In the alveolar macrophages and neutrophils, decreased NF-κBp65 and inducible nitric oxide synthase and increased I-κBα immunoreaction were detected in Platycodi Radix pretreated rats compared with LPS alone treated ones.Conclusion : It may be concluded that Platycodi Radix attenuates the development of LPS-induced inflammation by reduction of NF-κBp65 activation and neutrophil-mediated acute lung injury. Platycodi Radix would be useful as a therapeutic agent for endotoxin-induced lung disease.

keywords


참고문헌

1

(1994) Regulation of biosynthesis of nitric oxide,

2

(1996) LPS-mediated NF-kappa beta activation in rat Kupffer cells can be induced independently of CD14,

3

(1998) Nitric oxide release from the liver surface to the intraabdominal cavity during acute endotoxemia in rats,

4

(1994) regulation and function of NF-kappa B,

5

(1999) Induction of tolerance to hemorrhagic or endotoxic shock involves activation of NF-┟B,

6

(1999) .Flickinger and M.S,

7

(1997) NF-kappa B as a frequent target for immunosuppressive and anti-inflammatory molecules,

8

(1999) Intracellular signaling in rat cultured vascular smooth muscle cells Roles of nuclear factor-┟B and p38 mitogen-activated protein kinase on tumor necrosis factor-┒ production,

9

(1997) Effect of calpain inhibitor I an inhibitor of the proteolysis of I┟B on the circulatory failure and multiple organ dysfunction caused by endotoxin in the rat,

10

(1999) Effect of acrolein on human alveolar macrophage NF-┟B activity,

11

(1999) independently of IFN-gamma plays a crucial role in the pathogenesis of a murine psoriasis-like skin disorder,

12

(1999) A paradigm in information transfer form membrane to nucleus available at http,

13

(1997) The role of nuclear factor-kappa B in cytokine gene regulation,

14

(1998) Hemorrhagic shock primes for increased expression of cytokine-induced neutrophil chemoattractant in the lung role in pulmonary inflammation following lipopolysaccharide,

15

(1996) Evidence for common mechanisms in the transcriptional control of type II nitric oxide synthase in isolated hepatocytes Requirement of NF-┟B activation after stimulation with bacterial cell wall products and phorbol esters,

16

(2000) The glutathione precursor L-2-oxothiazolidine -4-carboxylic acid protects against liver injury due to chronic enternal ethanol exposure in the rat,

17

(1997) Impaired activation of nuclear factor-kappaB in endotoxin-tolerant rats is associated with down-regulation of chemokine gene expression and inhibition of neutrophilic lung inflammation,

18

(1999) Differential NF-kappaB activation after intratracheal endotoxin,

19

(1994) Cytokine-induced neutrophil chemoattractant mediates neutrophilic alveolitis in rats Association with nuclear factor kappa B activation,

20

(1998) Lipopolysaccharide-induced NF-kappaB activation and cytokine release in human alveolar macrophages is PKC-independent and TK- and PC-PLC-dependent,

21

(1998) Inhibition of inducible nitirc oxide synthase prevents LPS-induced acute lung injury in dogs,

  • 다운로드 수
  • 조회수
  • 0KCI 피인용수
  • 0WOS 피인용수

권호 내 다른 논문

추천 논문

상단으로 이동

대한한의학회지