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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Apoptotic Effects of Sipimikwanjung-tang of?Sasang Constitutional Medicine in Human Hepatoblastoma Cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.1, pp.46-58










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Abstract

Objectives : This study on the antioxidant effect of Sipimikwanjung-tang were undertaken to evaluate the antioxidant capacities and antiperoxidation activities in rat liver tissues. Sipimikwanjung-tang which has been one of the prescription in Sasang constitutional medicine is usually applied for the therapy of various liver diseases. It is elucidated that Sipimikwanjung-tang has the antioxidants on liver tissue of rat and the cytotoxic effects on human hepatoblastoma Hep G2 cells. Methods : Sipimikwanjung-tang extract in antioxidant effects of Hep G2 cells is evalated by MTT assay, DAPI staining, DNA fragmentation assays and FACScan analysis. Results : Sipimikwanjung-tang induced apoptosis in Hep G2 cells, and induced G1 and G2M arrest of the cell cycle as well as a significant increase in PARP and caspase-3 activity. Sipimikwanjung-tang induced an increase in H2O2 generation and the subsequent NF-κB activation and also induced cell apoptosis through the caspase-3-dependent pathways in the low concentration of Sipimikwanjung-tang extracts. However, the high dose of Sipimikwanjung-tang extract in Hep G2 cells inhibits TGF-β1-induced apoptosis via increase in cellular H2O2 formation and NF-κB activation in human hepatoblastoma Hep G2 cells. Conclusions : From this study, The possibility that Sipimikwanjung-tang extracts apply to antioxidentic and apoptotic treatment needed disease is revealed.

keywords
Sipimikwanjung-tang, SKT, hepatoblastoma cell, Hep G2 cell, caspase-3, TGF-β1, NF-κB, Sipimikwanjung-tang, SKT, hepatoblastoma cell, Hep G2 cell, caspase-3, TGF-β1, NF-κB


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