- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Effects of Kamijihwang-tang on Immune Cells andCytokines in OVA-induced Asthmatic Mice
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.2, pp.7-20
&
(2008). Effects of Kamijihwang-tang on Immune Cells andCytokines in OVA-induced Asthmatic Mice. Journal of Korean Medicine, 29(2), 7-20.
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Abstract
Objectives: The purpose of this study wasto examine the effects of Kamijihwang-tang (KJHT) extracts on immune cells and cytokines in ovalbumin (OVA)-induced asthmatic mice. Methods: C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four times a week) for asthma induction. Two experimental groups were treated with different concentrations of KJHT (400 ㎎/㎏ and 200 ㎎/㎏) extracts and cyclosporine A (10 ㎎/㎏) for the later 8 weeks. At the end of the experiment, the mice lung, PLN and spleen were removed and immune cells were analyzed by flow cytometer, IL-5, IL-13, eotaxin-2, TNF-α were analyzed by real-time PCR, serum histamine was analyzed by ELISA kit. Results: CD3+, CD3e-/CCR3+, CD3e+/CD69+, CD4+/CD25+, B220+/IgE+, and CD3e+/DX5+ cells in lung, PLN, and spleen of the KJHT group (400 ㎎/㎏) decreased compared with that of the control group. CD3e+/CD69+, CD4+/CD25+, and CD3e+/DX5+ cells in lung, PLN, and spleen of the KJHT group (200 ㎎/㎏), CD3+, CD3e-/CCR3+ cells in lung and PLN of the KJHT group (200 ㎎/㎏) and B220+/IgE+ cells in lung and spleen of the KJHT group (200 ㎎/㎏) decreased compared with that of the control group. mRNA expression of IL-5, IL-13, eotaxin-2, and TNF-α in lung tissue of the KJHT groups (400 ㎎/㎏ and 200 ㎎/㎏) decreased compared with that of the control group. Histamine in serum of the KJHT group (400 ㎎/㎏) decreased compared with that of the control group. Conclusions: These results suggest that KJHT can be utilized effectively in treating asthma because it significantly reduces inflammatory cells and cytokines.
- keywords
- Asthma, Kamijihwang-tang (KJHT), immune cells, cytokine
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