In 2016, as a subset study to discover indigenous prokaryotic species in Korea, a total of 15 bacterial strains were isolated and assigned to the class Betaproteobacteria. From the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 15 species have been described in Korea; therefore, 1 strain of the Aquitalea, 5 strains of the Paraburkholderia, 2 strains of the Comamonas, 1 strain of the Cupriavidus, 1 strain of the Diaphorobacter, 2 strains of the Hydrogenophaga, 1 strain of the Iodobacter, 1 strain of the Massilia and 1 strain of the Rhodoferax within the Betaproteobacteria are described for unreported bacterial species in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.
Doetsch, R.N. 1981. Determinative methods of light microscopy. Manual of Methods for General Bacteriology, pp. 21-33. In: P. Gerhardt, R.G.E. Murray, R.N. Costilow, E.W. Nester, W.A. Wood, N.R. Krieg and G.H. Phillips (eds.), American Society for Microbiology. Washington, DC, USA.
Euzeby, J.P. 2016. List of Prokaryotic Names with Standing in Nomenclature, as of February 2016 (www.bacterio.net).
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
Garrity, G.M., J.A. Bell and T. Lilburn. 2005. Class II. Betaproteobacteria class. nov. In: D.J. Brenner, N.R. Krieg, J.T. Staley and G.M. Garrity (eds.), Bergey'sManual of Systematic Bacteriology, second edition, vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria), Springer, New York, 2005, p. 575.
Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870-1874.
Martin, F., S. Torelli, D. Le Paslier, A. Barbance, F. Martin-Laurent, D. Bru, R. Geremia, G. Blake and Y. Jouanneau (2012). Betaproteobacteria dominance and diversity shifts in the bacterial community of a PAH-contaminated soil exposed to phenanthrene. Environmental Pollution 162: 345-353.
Nakatsu, C.H., K. Hristova, S. Hanada, X.Y. Meng, J.R. Hanson, K.M. Scow and Y. Kamagata. 2006. Methylibium petroleiphilum gen. nov., sp. nov., a novel methyl tert-butyl ether-degrading methylotroph of the Betaproteobacteria. International Journal of Systematic and Evolutionary Microbiology 56(5):983-989.
Pruesse, E., J. Peplies and F.O. Glockner. 2012. SINA: accurate highthroughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823-1829
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J. Lane. 1991. 16S ribosomal DNA amplifcation for phylogenetic study. Journal of Bacteriology 173:697-703.
Woese, C.R. 1987. Bacterial evolution. Microbiological reviews 51:221-271.
Yoon, S.H., S.M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. International Journal of Systematic and Evolutionary Microbiology 67:1613-1617.