open access
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Results from the research question should be reliable and valid, and depend on the scientific design of the study. For results with high precision and no bias, the study should proceed carefully according to a detailed protocol at each stage, including study conducting, data analysis, interpretation, etc., throughout the study period. Statistical design is required to lead the scientific design. Since fallacies at the design stage cannot be completely overcome by statistical adjustment procedures during the study or after the end of the study, full consideration of the whole process is required before the start of the study. In this study, we introduce several key items required in design stage. Statistical problems to be considered are discussed with case examples.
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