- P-ISSN 2233-4203
- E-ISSN 2093-8950
- 권한신청
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
논문 상세
Home > 논문 상세
Characterization of the Fragmentation Pattern of Peptide from Tandem Mass Spectra
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.2, pp.50-55
https://doi.org/10.5478/MSL.2019.10.2.50
(Cochin University of Science and technology)
(Cochin University of Science and technology)
(Cochin University of Science and technology)
Ramachandran,
S.,
&
Thomas,
T.
(2019). . Mass Spectrometry Letters, 10(2), 50-55, https://doi.org/10.5478/MSL.2019.10.2.50
- 다운로드 수
- 조회수
Abstract
The fragmentation statistics of ion trap CID (Collision-Induced Dissociation) spectra using 87,661 tandem mass spectra of doubly charged tryptic peptides are analyzed here. In contrast to the usual method of using intensity information, the frequency of occurrence of fragment ions, with respect to the position of the cleavage site and the residues at these sites is stud-ied in this paper. The analysis shows that the frequency of occurrence of fragment ion peaks is more towards the middle of the peptide than its ends. It was noted that amino acid with an aromatic and basic side chain at N- & C- terminal end of the peptide stimulates more peaks at the lower end of the spectrum. The residue pair effect was shown when the amide bond occurs between acidic and basic residues. The fragmentation at these sites (D/E-H/R/K) stimulates the generation of the y-ion peak. Also, the cleavage site H–H/R/K stimulates the generation of b-ions. K-P environment in the peptide sequence has more tendency to gen-erate y-ions than b-ions. Statistical analysis helps in the visualization of the CID fragmentation pattern. Cleavage pattern along the length of the peptide and the residue pair effects, enhance the knowledge of fragmentation behavior, which is useful for the better interpretation of tandem mass spectra.
- keywords
- Proteomics, MS/MS, CID, fragmentation pattern, residue dependence
참고문헌
1
Aebersold, R. (2003). . Nature, 422, 198-.
2
Diament, B. (2011). . J. Proteomics, 10, 3871-.
3
Dancík, V. (1999). . J. Comput. Biol, 6, 327-.
4
Huang, Y. (2008). . J. Proteome Res, 7, 70-.
5
Wysocki, V. H. (2000). . J. Mass Spectrom, 35, 1399-.
6
Boyd, R. (2010). . J. Am. Soc. Mass Spectrom, 21, 1275-.
7
Kapp, E. A. (2003). . Anal. Chem, 75, 6251-.
8
Elias, J. E. (2004). . Nat. Biotechnol, 22, 214-.
9
Huang, Y. (2005). . Anal. Chem, 77, 5800-.
10
Tabb, D. L. (2003). . Anal. Chem, 75, 1155-.
11
Martin, D. B. (2005). . Anal. Chem, 77, 4870-.
12
Schütz, F. (2003). . Biochem. Soc. Trans, 31, 1479-.
13
Raulfs, M. D. M. (2014). . J. Am. Soc. Mass Spectrom, 25, 1705-.
14
15
Rodriguez, J. (2008). . J. Proteome Res, 7, 300-.
16
Lau, K. W. (2009). . Rapid Commun. Mass Spectrom, 23, 1508-.
17
Allen, J. M. (2008). . J. Am. Soc. Mass Spectrom, 19, 1764-.
18
Medzihradszky, K. F. (2015). . Mass Spectrom. Rev, 34, 43-.
19
Khatun, J. (2007). . Anal. Chem, 79, 3032-.
20
Loo, J. A. (1993). . Anal. Chem, 65, 425-.
- 투고일Submission Date
- 2019-03-27
- 수정일Revised Date
- 2019-04-26
- 게재확정일Accepted Date
- 2019-05-08
권호 내 다른 논문
- Determination of Ceftiofur Residues by Simple Solid Phase Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry in Eel, Flatfish, and Shrimp
- Characterization of the Fragmentation Pattern of Peptide from Tandem Mass Spectra
- A Sensitive and Rapid LC-MS/MS Method for Determination of Berberine in Human Plasma
- Characterization of the Interaction between White Ginseng Extract and Selegiline Using Triple Quadrupole-Mass Spectrometry
- A Simple and Efficient Method to Determine Rivaroxaban in Rat Plasma Using Liquid-Liquid Extraction and LC-MRM
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.