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- E-ISSN 2093-8950
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- P-ISSN2233-4203
- E-ISSN2093-8950
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A Sensitive and Rapid LC-MS/MS Method for Determination of Berberine in Human Plasma
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.2, pp.56-60
https://doi.org/10.5478/MSL.2019.10.2.56
(Kangwon National University)
(Kangwon National University)
(Caleb Multilab Inc.)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Caleb Multilab Inc.)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
(Kangwon National University)
Kim,
J. H., Mai,
X., Kim,
K. Y., Sim,
M., Lee,
S., Seo,
H., Lee,
G., Kim,
D. J., &
Kim,
K.
H.
(2019). A Sensitive and Rapid LC-MS/MS Method for Determination of Berberine in Human Plasma. Mass Spectrometry Letters, 10(2), 56-60, https://doi.org/10.5478/MSL.2019.10.2.56
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Abstract
Coptidis Rhizoma (CR) has been used widely in traditional medicine to treat common diseases. This study aimed to develop a high-sensitivity liquid chromatography-tandem mass (LC-MS) spectrometry method for the evaluation of the pharmacokinetics of a new natural product that contain CR extract with the main bioactive compound, berberine, at trace concentrations. Human plasma samples were pretreated with methanol by a protein precipitation method. Berberine was analyzed on a Kinetex C18 column (2.1 mm × 50 mm, 100 Å, 1.7 µm) using a mobile phase of 10 mM ammonium formate/0.1% formic acid in water (A) and acetonitrile (B) (50:50, v/v) with a flow rate of 0.25 mL/min. The analyte was detected by using electrospray ionization in positive mode with multiple reaction monitoring (MRM). The method was sensitive, with a lower limit of quantification of 1 pg/mL, which has not been previously obtained. The method was validated (over the range of 1–50 pg/mL) and applied successfully for the pharmacokinetic study of human plasma samples.
- keywords
- Coptidis Rhizoma, berberine, human plasma, LC-MS/MS, pharmacokinetic study
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- Submission Date
- 2019-04-18
- Revised Date
- 2019-06-07
- Accepted Date
- 2019-06-13
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