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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Simultaneous Determination of α-Amanitin and β-Amanitin in Mouse Plasma Using Liquid Chromatography-High Resolution Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.3, pp.112-117
Bang Young Yoon (The Catholic University of Korea)
Lee Min Seo (The Catholic University of Korea)
Lim Chang Ho (The Catholic University of Korea)
Lee Hye Suk (The Catholic University of Korea)
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α-Amanitin and β-amanitin are highly toxic bicyclic octapeptides responsible for the poisoning of poisonous mush-rooms such as Amanita, Galerina, and Lepiota by inhibiting RNA polymerase II, DNA transcription, and protein synthesis. A sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using parallel reaction moni-toring mode was developed and validated for the simultaneous determination of α- and β-amanitin in mouse plasma to evaluate the toxicokinetics of α- and β-amanitin in mice. Protein precipitation of 5 μL mouse plasma sample with methanol as sample clean-up procedure and use of negative electrospray ionization resulted in better sensitivity and less matrix effect. The calibra-tion curves for α- and β-amanitin in mouse plasma were linear over the range of 0.5–500 ng/mL. The intra- and inter-day coeffi- cient of variations and accuracies for α- and β-amanitin at four quality control concentrations were 3.1–14.6% and 92.5–115.0%, respectively. The present method was successfully applied to the toxicokinetic study of α- and β-amanitin after an oral adminis-tration of α- and β-amanitin at 1.5 mg/kg dose to male ICR mice.

α-amanitin, β-amanitin, LC-HRMS, mouse plasma

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Mass Spectrometry Letters