ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Facile and Rapid Glycosylation Monitoring of Therapeutic Antibodies Through Intact Protein Analysis

Facile and Rapid Glycosylation Monitoring of Therapeutic Antibodies Through Intact Protein Analysis

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.3, pp.85-92
https://doi.org/10.5478/MSL.2021.12.3.85
Myung Jin Oh (Chungnam National University)
Nari Seo (Chungnam National University)
JungA Seo (Chungnam National University)
Ga Hyeon Kim (Chungnam National University)
Hyun Joo An (Chungnam National University)
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Abstract

The therapeutic antibody drug market has experienced explosive growth as mAbs become the main therapeutic modality for a variety of diseases. Characterization of glycosylation that directly affects the efficacy and safety of therapeutic monoclonal antibodies (mAbs) is critical for therapeutics development, bioprocess system optimization, lot release, and compa-rability evaluation. The LC/MS approach has been widely used to structurally characterize mAbs, and recently attempts have been made to obtain comprehensive information on the primary structure and post-translational modifications (PTMs) of mAbs through intact protein analysis. In this study, we performed state-of-the-art LC/MS based intact protein analysis to readily iden-tify and characterize glycoforms of various mAbs. Different glycoforms of mAbs produced in different expression cell lines includ- ing CHO, SP2/0 and HEK cells were monitored and compared. In addition, the comparability of protein molecular weight, glycoform pattern, and relative abundances of glycoforms between the commercialized trastuzumab biosimilar and the original product was determined in detail using the given platform. Intact mAb analysis allowed us to gain insight into the overall mAb structure, including the complexity and diversity of glycosylation. Furthermore, our analytical platform with high reproducibility is expected to be widely used for biopharmaceutical characterization required at all stages of drug development and manufacturing.

keywords
intact protein, glycoform monitoring, mass spectrometry, bioterapeutics


투고일Submission Date
2021-08-13
수정일Revised Date
2021-08-27
게재확정일Accepted Date
2021-09-08
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Mass Spectrometry Letters