• P-ISSN2233-4203
  • E-ISSN2093-8950

Article Detail

Home > Article Detail
  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Evaluation of the inhibitory effect of Gynostemma pentaphyllum extracts on CYP450 enzyme activities using LC-MS/MS

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2023, v.14 no.3, pp.116-119
Yu Jun Sang (Hanyang University)
Ji Young Seok (Hanyang University)
Jo So Young (Hanyang University)
Piao Xiang-Lan (Minzu University of China)
Yoo Hye Hyun (Hanyang University)
  • Downloaded
  • Viewed


Gynostemma pentaphyllum (Thunb.) Makino extract, a natural product with a history of traditional use, has gained attention for its potential health benefits. This study aimed to investigate its effects on key cytochrome P450 (CYP) enzymes using LC-MS/MS. Human liver microsomes and cDNA-expressed CYP2C8, CYP2C9, CYP2C19, and CYP3A4 supersomes were employed. Enzyme activity was assessed based on the formation of CYP-specific marker metabolites. The resulting data showed that the extract exhibited inhibitory effects on CYP2C8, CYP2C9, CYP2C19, and CYP3A4. Thus, G. pentaphyllum extract may influence the pharmacokinetics of drugs metabolized by CYP2C8, CYP2C9, CYP2C19, and CYP3A4. These find- ings emphasize the importance of considering potential herb-drug interactions when incorporating this extract into therapeutic regimens or dietary supplements.

Gynostemma pentaphyllum cytochrome P450 herb-drug interaction LC-MS/MS

Submission Date
Revised Date
Accepted Date
상단으로 이동

Mass Spectrometry Letters