- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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Estimation and Analysis Methods for Trastuzumab Deamidation Levels Using Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2024, v.15 no.2, pp.107-119
https://doi.org/10.5478/10.5478/MSL.2024.15.2.107
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Chungbuk National University)
(Chungbuk National University)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Binex Co., Ltd.)
(Chungbuk National University)
(Chungbuk National University)
Moon,
D.,
Kim,
G.,
Park,
M.,
Hong,
S.,
Nam,
M.,
Park,
S.,
&
Hong,
J.
(2024). . Mass Spectrometry Letters, 15(2), 107-119, https://doi.org/10.5478/10.5478/MSL.2024.15.2.107
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Abstract
We aimed to develop a suitable quantification method for detecting asparagine deamidation and aspartic acid isomer- ization in peptide mapping using LC-MS. Our assessment of its validity and suitability involved comparing its quantitative find- ings with those obtained from cation-exchange chromatography and capillary electrophoresis methods. By subjecting trastuzumab to rigorous conditions to induce these modifications, we validated the efficacy of this new analytical method in pep- tide mapping via LC-MS, evaluating both qualitative and quantitative aspects of asparagine deamidation and aspartic acid isom- erization. Our investigation underscored the significance of enzyme selection and the presence of miss-cleaved or non-specific peptides in achieving accurate quantitative results. The experimental results demonstrated a strong correlation with results from cation-exchange chromatography and capillary electrophoresis analyses, confirming the reliability of the LC-MS based peptide mapping approach.
- keywords
- mass spectrometry, quantification method, asparagine deamidation, Trastuzumab, aspartic acid isomerization, pep- tide mapping, tandem mass, CID, ETD
- 투고일Submission Date
- 2024-05-23
- 수정일Revised Date
- 2024-06-20
- 게재확정일Accepted Date
- 2024-06-20
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