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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Estimation and Analysis Methods for Trastuzumab Deamidation Levels Using Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2024, v.15 no.2, pp.107-119
Moon Daebong (Binex Co., Ltd.)
Kim Geonwoo (Binex Co., Ltd.)
Park Minjae (Binex Co., Ltd.)
Hong Sunyeol (Binex Co., Ltd.)
Nam Mihyeon (Binex Co., Ltd.)
Park Sungsic (Chungbuk National University)
Hong Jintae (Chungbuk National University)
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We aimed to develop a suitable quantification method for detecting asparagine deamidation and aspartic acid isomer- ization in peptide mapping using LC-MS. Our assessment of its validity and suitability involved comparing its quantitative find- ings with those obtained from cation-exchange chromatography and capillary electrophoresis methods. By subjecting trastuzumab to rigorous conditions to induce these modifications, we validated the efficacy of this new analytical method in pep- tide mapping via LC-MS, evaluating both qualitative and quantitative aspects of asparagine deamidation and aspartic acid isom- erization. Our investigation underscored the significance of enzyme selection and the presence of miss-cleaved or non-specific peptides in achieving accurate quantitative results. The experimental results demonstrated a strong correlation with results from cation-exchange chromatography and capillary electrophoresis analyses, confirming the reliability of the LC-MS based peptide mapping approach.

mass spectrometry, quantification method, asparagine deamidation, Trastuzumab, aspartic acid isomerization, pep- tide mapping, tandem mass, CID, ETD

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Mass Spectrometry Letters