- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
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Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.2, pp.53-56
https://doi.org/10.5478/MSL.2011.2.2.053
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
Song,
S., Yang,
H., Kim,
J. H., Shin,
S., Park,
E. H., &
Kim,
J.
(2011). Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis. Mass Spectrometry Letters, 2(2), 53-56, https://doi.org/10.5478/MSL.2011.2.2.053
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Abstract
C8-functionalized magnetic nanoparticles were synthesized by coating magnetic Fe3O4 nanoparticles with silica–amine groups using 3-aminopropyltriethoxysilane and by subsequently modifying the amine groups with chloro(dimethyl)octylsilaneto produce octyl groups on the surface of the MNPs. The C8-functionalized MNPs were used to enrich peptides from trypticprotein digests of myoglobin and α-casein. The enriched peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI−ΤΟF−MS). MALDI-MS was also used to investigate desalting of the C8-functionalized MNPs. Sample solutions were prepared in 1.0 M NaCl, and the successful removal of salt was observed. Enrichmentwith C8-functionalized MNPs was very effective for separating and concentrating tryptic peptides.
- keywords
- 3-aminopropyltriethoxysilane, chloro(dimethyl)octylsilane, MALDI, magnetic nanoparticles
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- Submission Date
- 2011-05-27
- Revised Date
- 2011-06-07
- Accepted Date
- 2011-06-07
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