- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
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Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.4, pp.83-86
https://doi.org/10.5478/MSL.2013.4.4.83
(Korea Research Institute of Standard and Science)
(Korea Research Institute of Standard and Science)
(Korea Research Institute of Standard and Science)
(Korea Research Institute of Standard and Science)
(Korea Research Institute of Standard and Science)
Yu,
H. D., Kim,
B., Shin,
D., &
Ahn,
S.
(2013). Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS). Mass Spectrometry Letters, 4(4), 83-86, https://doi.org/10.5478/MSL.2013.4.4.83
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Abstract
A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silicacapillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEFwas completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhancedthe ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying werewell completed with the new interface and the new spray needle.
- keywords
- CIEF-ESI-MS, Interface, Carbonic anhydrase I, Fourier transform mass spectrometry
Reference
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- Submission Date
- 2013-12-11
- Revised Date
- 2013-12-19
- Accepted Date
- 2013-12-19
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