ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.4, pp.83-86
https://doi.org/10.5478/MSL.2013.4.4.83
Hai Dong Yu (Korea Research Institute of Standard and Science)
Byungjoo Kim (Korea Research Institute of Standard and Science)
Dae-Ho Shin (Bio Medical Technologies Ltd)
Seonghee Ahn (Korea Research Institute of Standard and Science)
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Abstract

A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silicacapillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEFwas completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhancedthe ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying werewell completed with the new interface and the new spray needle.

keywords
CIEF-ESI-MS, Interface, Carbonic anhydrase I, Fourier transform mass spectrometry


참고문헌

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투고일Submission Date
2013-12-11
수정일Revised Date
2013-12-19
게재확정일Accepted Date
2013-12-19
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Mass Spectrometry Letters