![](/msletters/assets/coverImage/J000011_cover.jpg)
- P-ISSN 2233-4203
- E-ISSN 2093-8950
A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silicacapillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEFwas completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhancedthe ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying werewell completed with the new interface and the new spray needle.
Righetti, P. G. (1983). Isoelectric Focusing: Theory, Methodology and Applications:Elsevier Biomedical Press.
Hjerten, S. (1985). . Journal of Chromatogr, 346, 265-.
Tang, Q. (1995). . Anal. Chem, 67, 3515-.
Tang, Q. (1997). . Anal. Chem, 69, 3177-.
Martinovic, S. (2000). . Anal. Chem, 72, 5356-.
Clarke, N. J. (2002). . Biomed. Chromatogr, 16, 287-.
Kuroda, Y. (2005). . J. Pharm. Biomed. Anal, 37, 423-.
Mokaddem, M. (2009). . Electrophoresis, 30, 4040-.
Smith, R. D. (1988). . Anal. Chem, 60, 436-.
Pleasance, S. (1992). . J. Chromatogr, 591, 325-.
Shen, Y. (2000). . J. Microcol. Separations, 12, 135-.