- P-ISSN 2233-4203
- E-ISSN 2093-8950
- Log In/Sign Up
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Optimization of Enzyme Digestion Conditions for Quantification of Glycated Hemoglobin Using Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2014, v.5 no.2, pp.52-56
https://doi.org/10.5478/MSL.2014.5.2.52
(Korea Research Institute of Standard and Science)
Jeong,
J.
(2014). Optimization of Enzyme Digestion Conditions for Quantification of Glycated Hemoglobin Using Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry. Mass Spectrometry Letters, 5(2), 52-56, https://doi.org/10.5478/MSL.2014.5.2.52
- Downloaded
- Viewed
Abstract
Glycated hemoglobin (HbA1c) is used as an index of mean glycemia over prolonged periods. This study describes anoptimization of enzyme digestion conditions for quantification of non-glycated hemoglobin (HbA0) and HbA1c as diagnosticmarkers of diabetes mellitus. Both HbA0 and HbA1c were quantitatively determined followed by enzyme digestion using isotopedilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) with synthesized N-terminal hexapeptides asstandards and synthesized isotope labeled hexapeptides as internal standards. Prior to quantification, each peptide was additionallyquantified by amino acid composition analysis using ID-LC-MS/MS via acid hydrolysis. Each parameter was consideredstrictly as a means to improve digestion efficiency and repeatability. Digestion of hemoglobin was optimized when using100 mM ammonium acetate (pH 4.2) and a Glu-C-to-HbA1c ratio of 1:50 at 37oC for 20 h. Quantification was satisfactorilyreproducible with a 2.6% relative standard deviation. These conditions were recommended for a primary reference method ofHbA1c quantification and for the certification of HbA1c reference material.
- keywords
- glycated hemoglobin, isotope dilution LC-MS/MS, enzyme digestion, diabetes mellitus
Reference
1
Sacks, D. B.. (2011). . Diabetes Care, 34, 518-.
2
Bi, J.. (2012). . Anal. Bioanal. Chem, 403, 549-.
3
Bry, L.. (2001). . Clin. Chem, 47, 153-.
4
Thevarajah, M.. (2009). . Clin. Biochem, 42, 430-.
5
Kobold, U.. (1997). . Clin. Chem, 43, 1944-.
6
Little, R. R.. (2001). . Clin. Chem, 47, 1985-.
7
Goodall, I.. (2005). . Clin. Biochem. Rev, 26, 5-.
8
Little, R. R.. (2011). . Clin. Chem, 57, 205-.
9
Jeppsson, J. O.. (2002). . Clin. Chem. Lab. Med, 40, 78-.
10
Finke, A.. (1998). . Clin. Chem. Lab. Med, 36, 299-.
11
Kaiser, P.. (2010). . Clin. Chem, 56, 750-.
12
Vesper, H. W.. (2005). . Rapid Commun. Mass Spectrom, 19, 2865-.
13
Hirokawa, K.. (2005). . Biotechnol. Lett, 27, 963-.
14
Jeppsson, J. O.. (1986). . Clin. Chem, 32, 1867-.
15
Jeong, J. S.. (2011). . J. Chromatogr. A, 1218, 6596-.
16
Weiss, M.. (1998). . J. Chromatogr. A, 795, 263-.
17
Drapeau, G. R.. (1976). . Methods Enzymol, 45, 469-.
18
van den Ouweland, J. M.. (2010). . Clin. Biochem, 43, 623-.
- Submission Date
- 2014-04-21
- Revised Date
- 2014-06-05
- Accepted Date
- 2014-06-05
Other articles from this issue
- Diagnostic Evaluation of Enzyme Activity Related to Steroid Metabolism by Mass Spectrometry-Based Steroid Profiling
- Simultaneous Determination of Five Porphyrins in Human Urine and Plasma Using High Performance Liquid Chromatography-Tandem Mass Spectrometry
- Tertiary Matrices for the Analysis of Polyethylene Glycols Using MALDI-TOF MS
- Optimization of Enzyme Digestion Conditions for Quantification of Glycated Hemoglobin Using Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry
- Accurate Measurement of Arsenic in Laver by Gravimetric Standard Addition Method Combined with High Resolution Inductively Coupled Plasma Mass Spectrometry
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.