- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2014, v.5 no.3, pp.70-78
https://doi.org/10.5478/MSL.2014.5.3.70
(Konkuk University)
(Konkuk University)
(Konkuk University)
(Konkuk University)
(Konkuk University)
Kim,
Y. J., Kim,
H., &
Noh,
K.
(2014). Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics. Mass Spectrometry Letters, 5(3), 70-78, https://doi.org/10.5478/MSL.2014.5.3.70
- Downloaded
- Viewed
Abstract
Microglia are the confined immune cells of the central nervous system (CNS). In response to injury or infection,microglia readily become activated and release proinflammatory mediators that are believed to contribute to microglia-mediatedneurodegeneration. In the present study, inflammation was induced in the immortalized murine microglial cell line BV-2 bylipopolysaccharide (LPS) treatment. We firstly performed phosphoproteomics analysis and phosphoinositide lipidomics analysiswith LPS activated microglia in order to compare phosphorylation patterns in active and inactive microglia and to detect the patternof changes in phosphoinositide regulation upon activation of microglia. Mass spectrometry analysis of the phosphoproteomeof the LPS treatment group compared to that of the untreated control group revealed a notable increase in the diversity ofcellular phosphorylation upon LPS treatment. Additionally, a lipidomics analysis detected significant increases in the amounts ofphosphoinositide species in the LPS treatment. This investigation could provide an insight for understanding molecular mechanismsunderlying microglia-mediated neurodegenerative diseases.
- keywords
- Microglia, Lipopolysaccharide (LPS), Neuroinflammation, Phosphoproteomics, Lipidomics, Phosphoinositide
Reference
1
Dheen, S. T.. (2007). . Curr. Med. Chem, 14, 1189-1197.
2
Streit, W. J.. (2004). . J. Neuroinflammation, 1, 14-.
3
Kim, S. U.. (2005). . J. Neurosci. Res, 81, 302-313.
4
Lu, D. Y.. (2007). . Br. J. Pharmacol, 151, 396-405.
5
McGeer, P. L.. (1988). . Neurology, 38, 1285-1291.
6
Block, M. L.. (2007). . Nat. Rev. Neurosci, 8, 57-69.
7
Tufekci, K. U.. (2011). . Parkinsons Dis, , 487450-.
8
Carson, M. J.. (2006). . Clin. Neurosci. Res, 6, 237-245.
9
Herbert, M. R.. (2005). . Neuroscientist, 11, 417-440.
10
Liu, B.. (2003). . J. Pharmacol. Exp. Ther, 304, 1-7.
11
Boulet, I.. (1992). . Oncogene, 7, 703-710.
12
Bhat, N. R.. (1998). . J. Neurosci, 18, 1633-1641.
13
Chen, C. C.. (1998). . J Biol. Chem, 273, 19424-19430.
14
Henn, A.. (2009). . ALTEX, 26, 83-94.
15
Han, D.. (2013). . Proteomics, 13, 2984-2988.
16
Han, D.. (2014). . BMC Genomics, 15, 95-.
17
Jeon, H.. (2010). . J. Neuroimmunol, 229, 63-72.
18
Wenk, M. R.. (2003). . Nat. Biotechnol, 21, 813-817.
19
Milne, S. B.. (2005). . J. Lipid Res, 46, 1796-1802.
20
Simonsen, A.. (2001). . Curr. Opin. Cell Biol, 13, 485-492.
21
Parker, P.. (2004). . J. Biochem. Soc. Trans, 32, 893-898.
22
Pettitt, T. R.. (2006). . J. Lipid. Res, 47, 1588-1596.
23
Lee, S. Y.. . Neurochem. Int., 57, 600-607.
24
Jin, R.. . Biochem. Biophys. Res. Commun., 399, 458-464.
25
Wisniewski, J. R.. (2009). . Nat. Methods, 6, 359-362.
26
Keller, A.. (2002). . Anal. Chem, 74, 5383-5392.
- Submission Date
- 2014-07-19
- Revised Date
- 2014-08-27
- Accepted Date
- 2014-09-12
Other articles from this issue
- A Simple Carbamidomethylation-Based Isotope Labeling Method for Quantitative Shotgun Proteomics
- Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics
- A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation
- In Vitro Inhibitory Effect of Licoricidin on Human Cytochrome P450s
- Degradation Efficiency and Characterization of Lincomycin by Electron Beam Irradiation
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.