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- E-ISSN 2093-8950
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MS Platform for Erythropoietin Glycome Characterization
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2015, v.6 no.3, pp.53-58
https://doi.org/10.5478/MSL.2015.6.3.53
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
Seo,
Y., Kim,
U., Oh,
M. J., Yun,
N. Y., &
An,
H.
J.
(2015). MS Platform for Erythropoietin Glycome Characterization. Mass Spectrometry Letters, 6(3), 53-58, https://doi.org/10.5478/MSL.2015.6.3.53
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Abstract
Recombinant erythropoietins (EPOs) are an important class of biotherapeutics that stimulate red blood cell produc- tion. The quality, safety, and potency of EPO variants are determined largely by their glycosylation, which makes up nearly half their mass. Thus, detailed glycomic analyses are important to assess biotherapeutic quality and establish the equivalency of bio- similar EPOs now coming to market. High-resolution mass spectrometry (MS) has recently emerged as the premier tool for gly- can analysis in EPOs. Using the accurate mass measurements provided by high-resolution MS, the compositions of even large, complex glycans can easily be determined. When combined with a nano-LC separation, differentiation of structural isomers also becomes a possibility. These components, together, provide a comprehensive picture of biotherapeutic glycosylation. In this review, we provide an overview of MS-based analytical platform for glycomic characterization of EPO biotherapeutics and bio- similars.
- keywords
- Mass spectrometry, Erythropoietin, Glycosylation, Glycomics
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- Submission Date
- 2015-08-14
- Revised Date
- 2015-09-01
- Accepted Date
- 2015-09-02
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