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- E-ISSN 2093-8950
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Analysis of Glycerol with Isolation of Endogenous Interferences using “Dilute and Shoot” Strategy and High-Resolution Mass Spectrometry in Human Urine for Antidoping Testing
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.4, pp.111-115
https://doi.org/10.5478/MSL.2016.7.4.000
(Korea Institute of Science and Technology)
(Doping Control Center, Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technolog)
(Doping Control Center, Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technolog)
Kim,
Y., Min,
H., Sung,
C., Park,
J., Son,
J., Lee,
K. M., Kim,
H. J., Lee,
J., Kwon,
O. S., &
Kim,
K.
H.
(2016). Analysis of Glycerol with Isolation of Endogenous Interferences using “Dilute and Shoot” Strategy and High-Resolution Mass Spectrometry in Human Urine for Antidoping Testing. Mass Spectrometry Letters, 7(4), 111-115, https://doi.org/10.5478/MSL.2016.7.4.000
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Abstract
Glycerol was identified and isolated from endogenous interferences during analysis of human urine using high-resolution mass spectrometry (HRMS) for doping control. Urinary sample preparation was simple; the samples were diluted with an organic solvent and then analyzed using a liquid chromatography-mass spectrometry (“dilute and shoot” method). Although the interfering ion peaks were observed at the similar retention time of glycerol, the inference could be identified by isolation with HRMS and further investigation. Thus, creatinine was identified as the endogenous interference for glycerol analysis and it also caused ion suppression resulting in the decrease of glycerol signal. This study reports the first identification and efficient isolation of endogenous interferences in human urine for “dilute and shoot” method. The information about ion suppression could be novel to prevent overestimation or a false result for antidoping analysis.
- keywords
- Mass spectrometry, Doping control, Dilute and shoot, Orbitrap, Glycerol analysis
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- Submission Date
- 2016-11-30
- Revised Date
- 2016-12-14
- Accepted Date
- 2016-12-19
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