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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Analysis of Glycerol with Isolation of Endogenous Interferences using “Dilute and Shoot” Strategy and High-Resolution Mass Spectrometry in Human Urine for Antidoping Testing

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.4, pp.111-115
https://doi.org/10.5478/MSL.2016.7.4.000
Yongseok Kim (Korea Institute of Science and Technology)
Hophil Min (Doping Control Center, Korea Institute of Science and Technology)
Changmin Sung (Korea Institute of Science and Technology)
Ju-Hyung Park (Korea Institute of Science and Technology)
Junghyun Son (Korea Institute of Science and Technology)
Kang Mi Lee (Korea Institute of Science and Technology)
Ho Jun Kim (Korea Institute of Science and Technology)
Jaeick Lee (Korea Institute of Science and Technolog)
Oh Seung Kwon (Korea Institute of Science and Technolog)
Ki Hun Kim (Korea Institute of Science and Technolog)
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Abstract

Glycerol was identified and isolated from endogenous interferences during analysis of human urine using high-resolution mass spectrometry (HRMS) for doping control. Urinary sample preparation was simple; the samples were diluted with an organic solvent and then analyzed using a liquid chromatography-mass spectrometry (“dilute and shoot” method). Although the interfering ion peaks were observed at the similar retention time of glycerol, the inference could be identified by isolation with HRMS and further investigation. Thus, creatinine was identified as the endogenous interference for glycerol analysis and it also caused ion suppression resulting in the decrease of glycerol signal. This study reports the first identification and efficient isolation of endogenous interferences in human urine for “dilute and shoot” method. The information about ion suppression could be novel to prevent overestimation or a false result for antidoping analysis.

keywords
Mass spectrometry, Doping control, Dilute and shoot, Orbitrap, Glycerol analysis


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투고일Submission Date
2016-11-30
수정일Revised Date
2016-12-14
게재확정일Accepted Date
2016-12-19
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