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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Global Absolute Quantitation of Proteins in Human Whole Saliva by nLC-QIMS-TOF Employing MSE

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2017, v.8 no.4, pp.114-118
https://doi.org/10.5478/MSL.2017.8.4.114
Ha Ra Cho (Dankook University)
Sung Giu Jin (Dankook University)
Jun Seo Park (Dankook University)
Han Sol Kim (Dankook University)
Yong Seok Choi (Dankook University)
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Abstract

While saliva can be considered as good biological fluid for monitoring biomarkers due to many advantages including its communication with blood and the non-invasive nature during its sampling, its applications to that purpose is still limited. As a part of efforts to expand the applications of saliva to the protein biomarker research, we carried out global absolute quantitation of proteins in human whole saliva (WS) by bottom-up proteomics techniques mainly based on nLC-Q-IMS-TOF employing MSE. From the analyses of a pooled WS sample collected from 22 healthy Korean volunteers, 93 proteins ranging from 5.89×101 ng/mL (immunoglobulin heavy chain) to 1.59×104 ng/mL (α-amylase 1) were confirmed. For the validation of the present results, human serum albumin in the same sample was quantitated by ELISA and its result was compared with that from the nLC-Q-IMS-TOF study. As a result, there was no significant difference between two results from individual approaches (1.18×104 ± 0.03×104 ng/mL from nLC-Q-IMS-TOF experiments vs. 1.23×104 ± 0.07×104 ng/mL from ELISA experiments, n=3, p=0.309). To our knowledge, this is the first global absolute quantitation of proteins in human whole saliva and information from the present study can be widely used as the first level reference for the discovery

keywords
Human whole saliva, global absolute protein quantitation, MSE, proteome


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투고일Submission Date
2017-11-30
수정일Revised Date
2017-12-26
게재확정일Accepted Date
2017-12-26
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Mass Spectrometry Letters