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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

MS-Based Technologies for the Study of Site-Specific Glycosylation

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2017, v.8 no.4, pp.69-78
https://doi.org/10.5478/MSL.2017.8.4.69
Kim Unyong (Chungnam National University, GLYCAN Co., Ltd.,)
Oh Myung Jin (Chungnam National University)
Lee Jua (Chungnam National University)
Hwang Hee Yeon (Chungnam National University)
An Hyun Joo (Chungnam National University)
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Abstract

Glycosylation, which is one of the most common post-translation modification (PTMs) of proteins, plays a variety of crucial roles in many cellular events and biotherapeutics. Recent advances have led to the development of various analytical methods employing a mass spectrometry for glycomic and glycoproteomic study. However, site-specific glycosylation analysis is still a relatively new area with high potential for technologies and method development. This review will cover current MSbased workflows and technologies for site-specific mapping of glycosylation ranging from glycopeptide preparation to MS analysis. Bioinformatic tools for comprehensive analysis of glycoprotein with high-throughput manner will be also included.

keywords
Glycosylation, Glycopeptide, Mass Spectrometry, Site-specific mapping, Bioinformatics


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Submission Date
2017-11-17
Revised Date
2017-12-18
Accepted Date
2017-12-18
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