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- E-ISSN 2093-8950
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Effects of Temperature and Acetonitrile on Microwave-Assisted Weak Acid Protein Hydrolysis
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2018, v.9 no.2, pp.46-50
https://doi.org/10.5478/MSL.2018.9.2.46
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
Nam,
M., Lee,
D., Kim,
Y., &
Kim,
J.
(2018). Effects of Temperature and Acetonitrile on Microwave-Assisted Weak Acid Protein Hydrolysis. Mass Spectrometry Letters, 9(2), 46-50, https://doi.org/10.5478/MSL.2018.9.2.46
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Abstract
The effects of temperature and acetonitrile (ACN) concentration on microwave-assisted weak-acid hydrolysis of pro- teins were investigated. Myoglobin was hydrolyzed for 1 h using 2% formic acid and a microwave with different concentrations of ACN (0, 5, and 10%) at various temperatures (50, 60, 70, 80, 90, and 100 o C). The numbers of peptides identified with each concentration of ACN were the same for each temperature. The greatest number of peptides (18 total) was obtained with hydro- lysis at 100 o C, and 6 of these were a result of additional removal of aspartic acid at the C-terminus. Hydrolysis at 80 o C resulted in 13 peptides, of which only 1 was generated by the additional removal of aspartic acid, and 12 were observed with hydrolysis at 100 o C. Our results demonstrate that microwave-assisted weak-acid hydrolysis of proteins can be performed successfully at 80 o C, which could be beneficial for limiting side reactions and generating larger peptide sequences.
- keywords
- Microwave, MALDI, Acid hydrolysis, Acetonitrile, Temperature
Reference
1
Miyeong Seo. (2012). Microwave-assisted Weak Acid Hydrolysis of Proteins. Mass Spectrometry Letters, 3(2), 47-49. http://dx.doi.org/10.5478/MSL.2012.3.2.47.
2
서미영. (2013). Weak Acid Hydrolysis of Proteins. Bulletin of the Korean Chemical Society, 34(1), 27-28. http://dx.doi.org/10.5012/bkcs.2013.34.1.27.
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Remily-Wood, E.. (2009). . J. Am. Soc. Mass Spectrom., 20, 2106-. http://dx.doi.org/10.1016/j.jasms.2009.07.007.
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- Submission Date
- 2018-02-21
- Revised Date
- 2018-03-03
- Accepted Date
- 2018-03-12
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