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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2018, v.9 no.2, pp.56-60
https://doi.org/10.5478/MSL.2018.9.2.56
Insu Song (Sogang University)
Jae-ung Lee (Sogang University)
Jaehyeon Baek (DiatechKorea)
Sangwon Cha (Hankuk University of Foreign Studies)
Sang Yun Han (Gachon University)
Han Bin Oh (Sogang University)
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Abstract

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a n +H] + , [z n +H] + , and [y n ] + -type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

keywords
FRIPS, MALDI-TOF/TOF, mass spectrometry, peptides, radical ion, radical-driven fragmentation


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투고일Submission Date
2018-06-02
수정일Revised Date
2018-06-12
게재확정일Accepted Date
2018-06-12
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